Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,853,9420AC54.5% 4.1 / 12.0 11V182G (GTA→GGA) ydjKMFS transporter
Reads supporting (aligned to +/- strand):  ref base A (5/0);  new base C (0/6);  total (5/6)
Fisher's exact test for biased strand distribution p-value = 2.16e-03
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

GCGCGGGGATTCAGG‑AAAGTAGCGCCAGGCCAGCGCCGTAGCGATAAGCGACAATATTGCA‑GGGATAAGCAGTTGTACCCGCCAGTTCCACTCTGCACTAATCAGCGGCGTGAGTCCCATCGCTATCAATGAACAGAGCGGATATGACCAGTTGCCAA  >  NZ_CP009273/1853866‑1854023
                                                                              |                                                                                 
gcgcGGGGATTCAGG‑AAAGTAGCGCCAGGCCAGCGCCGTAGCGATAAGCGACAATATTGCA‑GGGATAAGCAGTTGTACCCGCCAGTTCCa                                                                      >  2:565240/1‑90 (MQ=255)
gcgcGGGGATTCAGG‑AAAGGAGCGCCAGGCCAGCGCCGTAGCGATAAGCGACAATATTGCA‑GGGATAAGCAGTTGTACCCGCCAGTTCCa                                                                      >  2:145966/1‑90 (MQ=255)
   gggggATTTAAGAAAAGGAGCGCCGGCCCGGCGCGGTAGGAAAAAGCAACAATATTGCG‑GGAATAAGCATTTGTCCCCGCCAGTTCCAct                                                                    <  2:281977/89‑1 (MQ=255)
   gggggATAAAGA‑AAAGGCGCGCCAGGCCAGCGCCGTAGCAAAAAGCAAAAATTTTGAG‑GGGAAAAGCATTTGTCCCCGCCAGTTCCActc                                                                   <  2:484312/89‑1 (MQ=255)
                aaaGTAGCGCCAGGCCAGCGCCGTAGCGATAAGCGACAATATTGCA‑GGGATAAGCAGTTGTACCCGCCAGTTCCACTCTGCACTAATCAg                                                       >  1:192714/1‑90 (MQ=255)
                      gcgcCAGGCCAGCGCCGTAGCGATAAGCGACAATATTGCAGGGGATAAGCAGTTGTACCCGCCAGTTCCACTCTGCACTAATCAgcggcg                                                  >  1:532891/1‑90 (MQ=255)
                                   cccGAAGCAAAAACCGAAAATATTGCG‑GGGAAAACCATTTGCCCCCGCCAGTTCCACTCTGCACTAATCAGCGGCGTGAGTCCCATCGCt                                    <  2:582055/89‑1 (MQ=255)
                                             aaaGCAACAATCTTGGG‑GGAAAAAGGAGTTCTCCCCGCCAGTTCCACTCTGCACTAATCAGCGGCGTGAGTCCCATCGCTATCAATGAAc                          <  2:285487/89‑1 (MQ=255)
                                                  aaaaaTATGGGA‑GGAATAAGTAGTTGCCCCCGCCAGTTCCACTCTGCACTAATCAGCGGCGTGAGTCCCATCGCTATCAATGAACAGAGc                     <  2:416044/87‑1 (MQ=255)
                                                            cA‑GGAAAAAGCATTTGTCCCCGCCAGTTCCACTCTGCACTAATCAGCGGCGTGAGTCCCATCGCTATCAATGAACAGAGCGGATATGAcc           <  2:769374/90‑1 (MQ=255)
                                                                      gCAGTTGTACCCGCCAGTTCCACTCTGCACTAATCAGCGGCGTGAGTCCCATCGCTATCAATGAACAGAGCGGATATGACCAGTTGCCaa  >  1:551984/1‑90 (MQ=255)
                                                                              |                                                                                 
GCGCGGGGATTCAGG‑AAAGTAGCGCCAGGCCAGCGCCGTAGCGATAAGCGACAATATTGCA‑GGGATAAGCAGTTGTACCCGCCAGTTCCACTCTGCACTAATCAGCGGCGTGAGTCCCATCGCTATCAATGAACAGAGCGGATATGACCAGTTGCCAA  >  NZ_CP009273/1853866‑1854023

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑