| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 3,806,432 | 0 | A | C | 64.7% | 8.2 / 14.1 | 17 | T114T (ACA→ACC) | coaBC | bifunctional phosphopantothenoylcysteine decarboxylase/phosphopantothenate‑‑cysteine ligase CoaBC |
| Reads supporting (aligned to +/- strand): ref base A (5/1); new base C (0/11); total (5/12) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 9.70e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.24e-04 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
TTCTCGCCCCTGCCACGGCAGATTTGATTGCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGT > NZ_CP009273/3806347‑3806509 | ttCTCGCCCCTGCCACGGCAGATTTGATTGCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTg < 1:348794/90‑1 (MQ=255) gATTTGATTGCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTcccc > 1:660576/1‑90 (MQ=255) gATTGCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCAt > 1:523393/1‑90 (MQ=255) aTTCCCCGTGTTGCTGCCGGAATGGCGAATGCCCGGGTACCGACGATTTGTCTGGCTACCCCTGCCCCTGTAGCCGTGCCCCCCGCCATg < 1:650846/90‑1 (MQ=255) cggtgtTGCTGCCGGAATGGCGAATGCCCTGGTATCGACGTTTTGTCTGGCTACCCCTGCGCCTGTAGCCGTGCCCCCCGCCATGAACca < 2:83488/88‑1 (MQ=255) ccGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTCCCCCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACca < 2:653006/90‑1 (MQ=255) tgcCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTa > 2:133327/1‑90 (MQ=255) gcCGGAAGGGCAAATGCCCTGGTATCGACGTTTTGTCTGCCTCCCCCTGCGCCTGTAGCCGTGCCCCCCGCCATGAACCAGCAGATGTAc < 1:346943/90‑1 (MQ=255) gAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTg > 2:346943/1‑90 (MQ=255) cccTGGTATCGACGATTTGTCTGGCTACCCCTGCCCCTGTAGCCGTGCCCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACgc < 2:298712/89‑1 (MQ=255) acttGGTACCGCCGATTTGTCTGGCTACCCCGGCCCCTTTAGCCGCCCTCCCCGCCATGACCCAGCAGATGTACCGTGCCGCTGCCACgc < 2:274350/87‑1 (MQ=255) ttaccgacgtTTTGCCTGGCTCCCCCTGCGCCTGTGGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCAt < 1:139320/86‑1 (MQ=255) gtttCGCCGTTTTGTCTGCCCCCCCCTGCGCTTGTAGCCGGGCCCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCAt < 1:615568/87‑1 (MQ=255) gTATCGACGTTTTGTCTGCCCCCCCCTGCGCCTGTAGCCGTGCCCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCAt < 1:673667/90‑1 (MQ=255) gTATCGACGTTTTGTCGGGCTACCCCGGCGCCTGTCGCCGTGCCCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCAt < 1:720448/90‑1 (MQ=255) gacgaTTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAAttt > 1:141162/1‑90 (MQ=255) ttGTCTGCCTCCCCCTGCGCCTGTGGCCGTCCCCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGt < 1:442364/90‑1 (MQ=255) | TTCTCGCCCCTGCCACGGCAGATTTGATTGCCCGTGTTGCTGCCGGAATGGCGAATGACCTGGTATCGACGATTTGTCTGGCTACACCTGCGCCTGTAGCCGTGCTCCCCGCCATGAACCAGCAGATGTACCGTGCCGCTGCCACGCAGCATAATTTAGAGGT > NZ_CP009273/3806347‑3806509 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |