| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 4,558,321 | 0 | T | G | 58.8% | 12.2 / 19.5 | 17 | T6P (ACC→CCC) | mdtM | multidrug efflux MFS transporter MdtM |
| Reads supporting (aligned to +/- strand): ref base T (0/7); new base G (10/0); total (10/7) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.14e-05 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.65e-04 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAAT > NZ_CP009273/4558235‑4558399 | aGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGaaa > 2:404070/1‑90 (MQ=255) ggCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGaaaa > 2:586405/1‑90 (MQ=255) ggCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGaaaa > 2:176597/1‑90 (MQ=255) gATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGaaaaaaaaa > 1:725519/1‑88 (MQ=255) cGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATaac < 1:185912/90‑1 (MQ=255) gTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAATGGGATaaaa > 1:293405/1‑88 (MQ=255) cAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAacaaacaa < 1:586405/90‑1 (MQ=255) gTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGgag‑a < 2:727008/90‑1 (MQ=255) aaaTCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAGTGGCATAAAAAAAAAATCCTTTTCCGGGG‑AAACCCAAc > 2:741830/1‑90 (MQ=255) aTCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAAAGGCAAAAAAAAAAAAACCTTGTCCGGAG‑AACCCCAACgg > 1:263603/1‑90 (MQ=255) aaCGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGGAAAAAAAAAAGGGATAAAAAAAAAAACCCTTTCCGGGGAAACCCCAAAgggg > 1:564579/1‑90 (MQ=255) aTCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AACCCCAACGGGGAAAACGa < 2:749309/90‑1 (MQ=255) gCGTGGCGGCATGGCGGGGAAAAAAACATGGCAAAACAAACAACTCCCTGTTCTGGG‑GACCCCAACGGGGGAAAAGAAAATTAATACCGt > 2:139541/1‑90 (MQ=255) gCGTGGCGGCATGGCGGGGAAAAAAAAAGGGGATAAAAAAAAAAACCTTTTCCGGGG‑GAACCCCACGGGGGAAAAGAAAATCAAAAACGt > 1:31322/1‑90 (MQ=255) cggcATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTaa < 1:353295/90‑1 (MQ=255) ggcATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTTAt < 1:561287/90‑1 (MQ=255) ggcATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAAt < 1:654798/90‑1 (MQ=255) | AGGCTGGATCAGATCCGTCGACAGATACGCAGCAAAGTCATACAAAATCAACGCCATCGGGAAAAACAGCGTGGCGGCATGGCGGGTAAAAAAACGTGGCATAACAAACAACTCCTTGTCCGGAG‑AACCCCAACGGGGAAAACGAAATTCAATACCGTCGGTAAT > NZ_CP009273/4558235‑4558399 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |