Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	49,736	A→T	intergenic (+105/‑87)	kefC → / → folA	glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	49,736	0	A	T	100.0%	102.3 / NA	28	intergenic (+105/‑87)	kefC/folA	glutathione‑regulated potassium‑efflux system protein KefC/type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (18/10);  total (18/10)

ACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAA  >  NZ_CP009273/49650‑49816                                                                                       |                                                                                 acacTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAtttt                                                                               <  2:22506/90‑1 (MQ=255)  cacTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATtttt                                                                              >  2:179073/1‑90 (MQ=255)  cacTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATtttt                                                                              >  2:618183/1‑90 (MQ=255)      ttACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTc                                                                          <  1:282828/90‑1 (MQ=255)      ttACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTc                                                                          <  1:573866/90‑1 (MQ=255)      ttACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTc                                                                          >  1:350595/1‑90 (MQ=255)                cTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGGGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATcc                                                                >  1:518175/1‑90 (MQ=255)                      gtttttgtttCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCAc                                                          <  2:77642/90‑1 (MQ=255)                                      aaTGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGttt                                          <  2:574321/90‑1 (MQ=255)                                      aaTGCGGCGAGACCAGGGAGAGAGCGTGTACTCACCAGCAGAATATAAATTTTTCCTTAACATCATCCTCGCACCAGTCGACGACGGttt                                          >  1:331358/1‑90 (MQ=255)                                           ggcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCt                                     <  1:191141/90‑1 (MQ=255)                                            gcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCtt                                    >  2:439042/1‑90 (MQ=255)                                            gcgAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCtt                                    >  2:334169/1‑90 (MQ=255)                                              gAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTa                                  <  1:346637/90‑1 (MQ=255)                                              gAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTa                                  >  1:94974/1‑90 (MQ=255)                                                    aGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGCCGACGACGGTTTACGCTTTACGtata                            >  1:36999/1‑90 (MQ=255)                                                         gagagCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGc                       >  2:732260/1‑90 (MQ=255)                                                         gagagCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGc                       >  2:183322/1‑90 (MQ=255)                                                          agagCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTAc                                        >  2:135913/1‑72 (MQ=255)                                                          agagCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTAc                                        <  1:135913/72‑1 (MQ=255)                                                          agagCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCg                      >  2:322886/1‑90 (MQ=255)                                                           gagCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCt                                     >  1:713296/1‑74 (MQ=255)                                                           gagCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCt                                     <  2:713296/74‑1 (MQ=255)                                                           gagCGTGGACTCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGa                     >  1:585821/1‑90 (MQ=255)                                                                     tCGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAAttttttt           >  1:157600/1‑90 (MQ=255)                                                                      cGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAAtttttttt          >  2:712832/1‑90 (MQ=255)                                                                      cGCCAGCAGAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAAtttttttt          >  2:746236/1‑90 (MQ=255)                                                                              gAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGaa  <  2:473139/90‑1 (MQ=255)                                                                              gAATATAAATTTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGaa  <  1:195438/90‑1 (MQ=255)                                                                                       |                                                                                 ACACTTTACAGCTAACTGTTTGTTTTTGTTTCATTGTAATGCGGCGAGTCCAGGGAGAGAGCGTGGACTCGCCAGCAGAATATAAAATTTTCCTCAACATCATCCTCGCACCAGTCGACGACGGTTTACGCTTTACGTATAGTGGCGACAATTTTTTTTATCGGGAA  >  NZ_CP009273/49650‑49816

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑