Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	139,618	A→G	E118G (GAA→GGA)	yadG →	ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	139,618	0	A	G	100.0%	156.6 / NA	44	E118G (GAA→GGA)	yadG	ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (19/25);  total (19/25)

AACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGAAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAGCGCCGTTTAA  >  NZ_CP009273/139533‑139701                                                                                      |                                                                                    aaCCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAg                                                                                 <  1:100905/90‑1 (MQ=255) aaCCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAg                                                                                 <  2:728756/90‑1 (MQ=255)  aCCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGGGCAAAGAAGCGTACATCCGCAGCGGAAAgg                                                                                >  2:446890/1‑89 (MQ=255)           aaaCCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAaac                                                                       <  2:709896/90‑1 (MQ=255)                gTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAaac                                                                       <  1:10568/85‑1 (MQ=255)                gTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAaac                                                                       >  2:10568/1‑85 (MQ=255)                gTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTc                                                                  >  2:481245/1‑90 (MQ=255)                 tGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCg                                                                 >  2:614781/1‑90 (MQ=255)                              ggtgAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGaaa                                                    <  2:423687/90‑1 (MQ=255)                                tgAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAAc                                                  <  2:280192/90‑1 (MQ=255)                                 gAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACg                                                 >  1:175394/1‑90 (MQ=255)                                   aTCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAg                                                                                 >  2:738362/1‑56 (MQ=255)                                   aTCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAg                                                                                 <  1:738362/56‑1 (MQ=255)                                    tCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTTCATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCaa                                              <  2:336037/90‑1 (MQ=255)                                          aggGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCaacgaacg                                        <  1:276863/90‑1 (MQ=255)                                           gggTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGt                                       <  2:690750/90‑1 (MQ=255)                                           gggTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGt                                       >  2:529722/1‑90 (MQ=255)                                           gggTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGt                                       <  2:273656/90‑1 (MQ=255)                                               actacGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTgcgc                                   <  2:540783/90‑1 (MQ=255)                                               actacGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTgcgc                                   <  2:769033/90‑1 (MQ=255)                                               actacGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTgcgc                                   <  1:63116/90‑1 (MQ=255)                                                ctacGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGgaa                                                                                   >  2:767579/1‑41 (MQ=255)                                                ctacGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGgaa                                                                                   <  1:767579/41‑1 (MQ=255)                                                ctacGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGcgcg                                  <  2:175394/90‑1 (MQ=255)                                                  acGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTa                                <  1:713509/90‑1 (MQ=255)                                                  acGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTa                                <  1:702418/90‑1 (MQ=255)                                                  acGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTa                                >  2:412908/1‑90 (MQ=255)                                                     gCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAaaca                                                                      >  2:599000/1‑49 (MQ=255)                                                     gCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAaaca                                                                      <  1:599000/49‑1 (MQ=255)                                                     gCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGt                                       <  2:266341/80‑1 (MQ=255)                                                     gCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGt                                       >  1:266341/1‑80 (MQ=255)                                                     gCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGt                             >  1:677835/1‑90 (MQ=255)                                                     gCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGt                             >  1:521045/1‑90 (MQ=255)                                                     gCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGt                             >  2:377720/1‑90 (MQ=255)                                                     gCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGt                             >  2:360066/1‑90 (MQ=255)                                                      cGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGcgcg                                  >  2:358284/1‑84 (MQ=255)                                                      cGTGGAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGcgcg                                  <  1:358284/84‑1 (MQ=255)                                                          gAGCGCAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCt                        >  2:295047/1‑90 (MQ=255)                                                               cAAAGAAGCGTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCgg                   <  1:481245/90‑1 (MQ=255)                                                                        gTACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAgcg          <  1:445604/90‑1 (MQ=255)                                                                         tACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAgcgc         <  2:589718/90‑1 (MQ=255)                                                                         tACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAgcgc         <  1:442983/90‑1 (MQ=255)                                                                         tACATCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAgcgc         <  1:39278/90‑1 (MQ=255)                                                                             tCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAGCGCCGtt     >  1:224264/1‑90 (MQ=255)                                                                             tCCGCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAGCGCCGtt     >  2:119015/1‑90 (MQ=255)                                                                                gCAGCGGAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAGCGCCGTTTaa  >  2:126755/1‑90 (MQ=255)                                                                                      |                                                                                    AACCCGTTTGAAACCGTGCAGCAAATTGTGGTGAATCAGGCAGGGTACTACGGCGTGGAGCGCAAAGAAGCGTACATCCGCAGCGAAAAGTATCTTAAACAACTCGATCTATGGGGAAAACGCAACGAACGTGCGCGTATGTTATCTGGCGGGATGAAGCGCCGTTTAA  >  NZ_CP009273/139533‑139701

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑