Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,818,956	T→G	K80T (AAA→ACA)	ves ←	environmental stress‑induced protein Ves

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,818,956	0	T	G	100.0%	21.2 / NA	8	K80T (AAA→ACA)	ves	environmental stress‑induced protein Ves
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base G (2/6);  total (2/6)

ATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCAT  >  NZ_CP009273/1818869‑1819032                                                                                        |                                                                             atgatgTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGtgtt                                                                            <  2:57059/90‑1 (MQ=255)      gTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTGTTAAGGt                                                                       <  1:666575/90‑1 (MQ=255)       ttGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTGTTAAGGTa                                                                      <  1:545799/90‑1 (MQ=255)                      aTCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTGTTAAGGTATGGTTAAAGCGGTCt                                                       >  1:406670/1‑90 (MQ=255)                        ctgccctgccGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTGTTAAGGTATGGTTAAAGCGGTCTgc                                                     <  1:75517/90‑1 (MQ=255)                                         tCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGGGTTAAGGTATGGGTAAAGCGGTCTGCGCTTTCAAGGAGCAtct                                    >  2:249823/1‑90 (MQ=255)                                                 accaccTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTGTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCt                            <  2:752536/90‑1 (MQ=255)                                                               tGCAAAGGCAAAAGGCTGGAACGGTGTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCAc              <  2:591249/90‑1 (MQ=255)                                                                           aGGCTGGAACGGTGTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCAt  <  1:697654/90‑1 (MQ=255)                                                                                        |                                                                             ATGATGTTGAAATCCATCGACATCTGCCCTGCCGTCAGTTTCGCTTTTACCACCTGGTCCGCTGCAAAGGCAAAAGGCTGGAACGGTTTTAAGGTATGGTTAAAGCGGTCTGCGCTTTCAAGGAGCATCTCGCCGCCTTCCAGCAACGTCACTATCCTTTCCAT  >  NZ_CP009273/1818869‑1819032

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑