Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,838,813	T→C	I106V (ATT→GTT)	ynjI ←	DUF1266 domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,838,813	0	T	C	100.0%	118.4 / NA	34	I106V (ATT→GTT)	ynjI	DUF1266 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (19/15);  total (19/15)

CCAGCGTTTCAGACCATTGCTTATGATAGAATCGATAAGGTGCGATCAATTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAATACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACAATAAGCCCCATCATAATTAATAG  >  NZ_CP009273/1838726‑1838894                                                                                        |                                                                                  ccAGCGTTTCAGACCATTGCTTATGATAGAATCGATAAGGTGCGATCAATTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAacac                                                                                 >  2:3530/1‑90 (MQ=255)      gTTTCAGACCATTGCTTATGATAGAATCGATAAGGTGCGATCAATTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTa                                                                            <  2:627028/90‑1 (MQ=255)           aGACCATTGCTTATGATAGAATCGATAAGGTGCGATCAATTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTg                                                                       <  1:37765/90‑1 (MQ=255)           aGACCATTGCTTATGATAGAATCGATAAGGTGCGATCAATTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTg                                                                       <  1:392155/90‑1 (MQ=255)             aCCATTGCTTATGATAGAATCGATAAGGTGCGATCAATTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTGat                                                                     <  2:179290/90‑1 (MQ=255)                 ttGCTTATGATAGAATCGATAAGGTGCGATCAATTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTGataata                                                                 >  1:55841/1‑90 (MQ=255)                        tGATAGAATCGATAAGGTGCGATCAATTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTGATAATAATGCAgt                                                          >  1:578316/1‑90 (MQ=255)                             gAATCGATAAGGTGCGATCAATTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTc                                                     >  1:395556/1‑90 (MQ=255)                             gAATCGATAAGGTGCGATCAATTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTc                                                     >  1:531511/1‑90 (MQ=255)                                       ggTGCGATCAATTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCt                                           >  2:624669/1‑90 (MQ=255)                                       ggTGCGATCAATTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCt                                           >  1:542039/1‑90 (MQ=255)                                            gATCAATTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTaaa                                      >  2:154258/1‑90 (MQ=255)                                                 aTTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGcc                                                                         >  1:104238/1‑50 (MQ=255)                                                 aTTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGcc                                                                         <  2:104238/50‑1 (MQ=255)                                                      aGCGCCTGTAACTTCTCGATACTGAGCGGCTCAACCCCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGa                            <  2:205143/90‑1 (MQ=255)                                                      aGCGCCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTTTTTTTTTGCTTTAAAACCGGCCCGa                            >  1:136531/1‑90 (MQ=255)                                                      aGCGCCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGa                            <  1:485181/90‑1 (MQ=255)                                                      aGCGCCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCAGa                            <  1:737214/90‑1 (MQ=255)                                                         gcCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACa                         >  2:548150/1‑90 (MQ=255)                                                         gcCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACa                         >  1:467049/1‑90 (MQ=255)                                                         gcCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACa                         >  2:472417/1‑90 (MQ=255)                                                         gcCTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACa                         >  1:320477/1‑90 (MQ=255)                                                           cTGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACAAt                       >  1:333151/1‑90 (MQ=255)                                                            tGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACAATa                      <  1:43833/90‑1 (MQ=255)                                                            tGTAACTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACAATa                      <  2:531511/90‑1 (MQ=255)                                                               aaCTTCTCGATACTGAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACAATAAGc                   <  2:333151/90‑1 (MQ=255)                                                                    ctcGATACTGAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACAATAAGCCCcat              <  2:578316/90‑1 (MQ=255)                                                                    ctcGATACTGAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACAATAAGCCCcat              <  1:136539/90‑1 (MQ=255)                                                                         tACTGAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACAATAAGCCCCATCAtaa         <  1:548150/90‑1 (MQ=255)                                                                             gAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACAATAAGCCCCATCAtaattaa     >  2:28772/1‑90 (MQ=255)                                                                             gAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACAATAAGCCCCATCAtaattaa     >  2:724386/1‑90 (MQ=255)                                                                             gAGCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACAATAAGCCCCATCAtaattaa     >  2:98623/1‑90 (MQ=255)                                                                             gAGCGGCTCAACACCTTTAGCCTGATAATAATGAAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACAATAAGCCCCATCAtaattaa     >  1:653316/1‑90 (MQ=255)                                                                               gCGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACAATAAGCCCCATCATAATTAATa   <  1:258705/90‑1 (MQ=255)                                                                                cGGCTCAACACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACAATAAGCCCCATCATAATTAATAg  >  1:404556/1‑90 (MQ=255)                                                                                        |                                                                                  CCAGCGTTTCAGACCATTGCTTATGATAGAATCGATAAGGTGCGATCAATTGTAGCGCCTGTAACTTCTCGATACTGAGCGGCTCAATACCTTTAGCCTGATAATAATGCAGTTGTTCTTTTTTTGCTTTAAAACCGGCCCGAACAATAAGCCCCATCATAATTAATAG  >  NZ_CP009273/1838726‑1838894

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑