Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,840,928	T→C	E97G (GAA→GGA)	topB ←	DNA topoisomerase III

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,840,928	0	T	C	100.0%	85.7 / NA	25	E97G (GAA→GGA)	topB	DNA topoisomerase III
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (9/16);  total (9/16)

CTTTTCCGGTGCCAGTTGCAGATAGTCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTTCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTCGGGGCTGTAATTGCCACTTTTCC  >  NZ_CP009273/1840843‑1841008                                                                                      |                                                                                 cTTTTCCGGTGCCAGTTGCAGATAGTCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCt                                                                              <  2:232888/90‑1 (MQ=255) cTTTTCCGGTGCCAGTTGCAGATAGTCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCt                                                                              <  1:756836/90‑1 (MQ=255)     tCCGGTGCCAGTTGCAGATAGTCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCt                                                                          <  1:680717/90‑1 (MQ=255)                 tGCAGATAGTCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAAc                                                              <  1:150288/90‑1 (MQ=255)                 tGCAGATAGTCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAAc                                                              <  2:532320/90‑1 (MQ=255)                 tGCAGATAGTCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAAc                                                              <  2:420431/90‑1 (MQ=255)                 tGCAGATAGTCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAAc                                                              <  1:710965/90‑1 (MQ=255)                        aatCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTtga                                                       <  1:469709/88‑1 (MQ=255)                         gTCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTtgat                                                      <  2:2217/90‑1 (MQ=255)                              gCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGt                                                 <  1:173375/90‑1 (MQ=255)                              gCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGt                                                 <  1:225183/90‑1 (MQ=255)                                   tCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAgt                                            >  2:710664/1‑90 (MQ=255)                                   tCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAgt                                            >  2:706182/1‑90 (MQ=255)                                   tCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAgt                                            >  2:613571/1‑90 (MQ=255)                                   tCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAgt                                            >  1:759858/1‑90 (MQ=255)                                   tCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAgt                                            >  1:684750/1‑90 (MQ=255)                                   tCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAgt                                            >  1:510932/1‑90 (MQ=255)                                   tCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAgt                                            >  1:142625/1‑90 (MQ=255)                                           caGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGt                                    >  1:694751/1‑90 (MQ=255)                                                      cTTCACGATCCGGGTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTc                         <  2:165662/90‑1 (MQ=255)                                                           cGATCAGGGTCCCCGGCGTGAAGGATTCCACTGGCTACAGGCAGGAACCGTTTGATGACGTTAAGTCGTTTGGTCACGCAGGGTCGGGGc                    <  1:115687/90‑1 (MQ=255)                                                                 gggTCCCCGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTCGGGGCTGTAAt              >  1:599087/1‑90 (MQ=255)                                                                        cGGCGTGGACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGCCACGGAGGGTCGGGGCTGTAATTGCCACt       <  1:363351/90‑1 (MQ=255)                                                                        cGGCGTGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTCGGGGCTGTAATTGCCACt       <  2:599087/90‑1 (MQ=255)                                                                             tGAACGATTCCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTCGGGGCTGTAATTGCCACTTTTcc  <  2:182742/90‑1 (MQ=255)                                                                                      |                                                                                 CTTTTCCGGTGCCAGTTGCAGATAGTCCAGCACTTCATCCACCAGCAATTGCCCTTCACGATCCGGGTCCCCGGCGTGAACGATTTCGCTGGCTTCATGCAGGAACCGTTTGATGACGTTAAGTTGTTTGGTCACGGAGGGTCGGGGCTGTAATTGCCACTTTTCC  >  NZ_CP009273/1840843‑1841008

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑