Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,854,126	T→C	M121V (ATG→GTG)	ydjK ←	MFS transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,854,126	0	T	C	100.0%	89.1 / NA	26	M121V (ATG→GTG)	ydjK	MFS transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (7/19);  total (7/19)

TCCACGATTTCTACCGGGCATATATTCGGTGAAACCAGCAAACAGCGTAACCAGTAAAGCTCCCAGCCCAACTCCCATCACAAAACGGCAGGCAATAAGGAAATCCATATT‑CGGTGAGAAAGCACCGACAACCATCGAGGCAATATGAATGGCC  >  NZ_CP009273/1854049‑1854202                                                                              |                                                                              tCCACGATTTCTACCGGGCATATATTCGGTGAAACCAGCAAACAGCGTAACCAGTAAAGCTCCCAGCCCAACTCCCACCACAAAACggca                                                                   <  1:468206/90‑1 (MQ=255)             aCCGGGCATATATTCGGTGAAACCAGCAAACAGCGTAACCAGTAAAGCTCCCAGCCCAACTCCCACCACAAAACGGCAGGCAATAAGGaa                                                       <  1:754416/90‑1 (MQ=255)             aCCGGGCATATATTCGGTGAAACCAGCAAACAGCGTAACCAGTAAAGCTCCCAGCCCAACTCCCACCACAAAACGGCAGGCAATAAGGaa                                                       <  2:415250/90‑1 (MQ=255)             aCCGGGCATATATTCGGTGAAACCAGCAAACAGCGTAACCAGTAAAGCTCCCAGCCCAACTCCCACCACAAAACGGCAGGCAATAAGGaa                                                       <  2:18155/90‑1 (MQ=255)              ccGGGCATATATTCGGTGAAACCAGCAAACAGCGTAACCAGTAAAGCTCCCAGCCCAACTCCCACCACAAAACGGCAGGCAATAAGGaaa                                                      >  1:55765/1‑90 (MQ=255)                gggCATATATTCGGTGAAACCAGCAAACAGCGTAACCAGTAAAGCTCCCAGCCCAACTCCCACCACAAAACGGCAGGCAATAAGGAAATc                                                    >  2:214182/1‑90 (MQ=255)                 ggCATATATTCGGTGAAACCAGCAAACAGCGTAACCAGTAAAGCTCCCAGCCCAACTCCCACCACAAAACGGCAGGCAATAAGGAAATcc                                                   >  1:546465/1‑90 (MQ=255)                             gTGAAACCAGCAAACAGCGTAACCAGTAAAGCTCCCAGCCCAACTCCCACCACAAAACGGCAGGCAATAAGGAAATCCATATT‑CGGTgag                                      >  2:193637/1‑90 (MQ=255)                                 aaCCAGCAAACAGCGTAACCAGTAAAGCTCCCAGCCCAACTCCCACCACAAAACGGCAGGCAATAAGGAAATCCATATT‑CGGTGAGAAAg                                  <  2:560518/90‑1 (MQ=255)                                          aCAGCGTAACCAGTAAAGCTCCCAGCCCAACTCCCACCACAAAACGGCAGGCAATAAGGAAATCCATATT‑CGGTGAGAAAGCACCGACaa                         <  2:142661/90‑1 (MQ=255)                                                tAACCAGTAAAGCTCCCAGCCCAACTCCCACCACAAAACGGCAGGCAATAAGGAAATCCATATT‑CGGTGAGAAAGCACCGACAACCATCg                   <  2:367004/90‑1 (MQ=255)                                                  aCCAGTAAAGCCCCCAGCCCAACTCCCACCACAAAACGGCAGGCAATAAGGAAATCCATATT‑CGGTGAGAAAGCACCGACAACCATCGAg                 <  2:625450/90‑1 (MQ=255)                                                   ccAGTAAAGCTCCCAGCCCAACCCCCACCACAAAACGGCAGGCAATAAGGAAATCCATATT‑CGGTGAGAAAGCACCGACAACCATCGAgg                <  1:579733/90‑1 (MQ=255)                                                     aGTAAAGCTCCCAGCCCAACTCCCACCACAAAACGGCAGGCAATAAGGAAATCCATATT‑CGGTGAGAAAGCACCGACaa                         <  2:571114/79‑1 (MQ=255)                                                     aGTAAAGCTCCCAGCCCAACTCCCACCACAAAACGGCAGGCAATAAGGAAATCCATATT‑CGGTGAGAAAGCACCGACaa                         <  2:31737/79‑1 (MQ=255)                                                     aGTAAAGCTCCCAGCCCAACTCCCACCACAAAACGGCAGGCAATAAGGAAATCCATATT‑CGGTGAGAAAGCACCGACaa                         >  1:571114/1‑79 (MQ=255)                                                     aGTAAAGCTCCCAGCCCAACTCCCACCACAAAACGGCAGGCAATAAGGAAATCCATATT‑CGGTGAGAAAGCACCGACaa                         >  1:31737/1‑79 (MQ=255)                                                             tCCCAGCCCAACTCCCACCACAAAACGGCAGGCAATAAGGAAATCCATATT‑CGGTGAGAAAGCACCGACAACCATCGAGGCAATATGAAt      <  2:78217/90‑1 (MQ=255)                                                             tCCCAGCCCAACTCCCACCACAAAACGGCAGGCAATAAGGAAATCCATATT‑CGGTGAGAAAGCACCGACAACCATCGAGGCAATATGAAt      <  2:66862/90‑1 (MQ=255)                                                             tCCCAGCCCAACTCCCACCACAAAACGGCAGGCAATAAGGAAATCCATATT‑CGGTGAGAAAGCACCGACAACCATCGAGGCAATATGAAt      <  1:686174/90‑1 (MQ=255)                                                             tCCCAGCCCAACTCCCACCACAAAACGGCAGGCAATAAGGAAATCCATATT‑CGGTGAGAAAGCACCGACAACCATCGAGGCAATATGAAt      <  1:655725/90‑1 (MQ=255)                                                             tCCCAGCCCAACTCCCACCACAAAACGGCAGGCAATAAGGAAATCCATATT‑CGGTGAGAAAGCACCGACAACCATCGAGGCAATATGAAt      <  1:515888/90‑1 (MQ=255)                                                              cccAGCCCAACTCCCACCACAAAACGGCAGGCAATAAGGAAATCCATATT‑CGGTGAGAAAGCACCGACAACCATCGAGGCAATATGAATg     >  1:523525/1‑90 (MQ=255)                                                                 aGCCCAACTCCCACCACAAAACGGCAGGCAATAAGGAAATCCATATT‑CGGTGAGAAAGCACCGACAACCATCGAGGCAATATGAATGGcc  <  2:532891/90‑1 (MQ=255)                                                                 aGCCCAACTCCCACCACAAAACGGCAGGCAATAAGGAAATCCATATT‑CGGTGAGAAAGCACCGACAACCATCGAGGCAATATGAATGGcc  <  1:401524/90‑1 (MQ=255)                                                                  gCCCAACTCCCACCACAAAACGGCAGGCAATAAGGAAATCCATATTCCGGTGAGAAAGCACCGACAACCATCGAGGCAATATGAATGGcc  <  2:103482/90‑1 (MQ=255)                                                                              |                                                                              TCCACGATTTCTACCGGGCATATATTCGGTGAAACCAGCAAACAGCGTAACCAGTAAAGCTCCCAGCCCAACTCCCATCACAAAACGGCAGGCAATAAGGAAATCCATATT‑CGGTGAGAAAGCACCGACAACCATCGAGGCAATATGAATGGCC  >  NZ_CP009273/1854049‑1854202

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑