| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 2,029,236 | T→C | intergenic (+512/‑80) | ompC → / → hchA | porin OmpC/protein deglycase HchA |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,029,236 | 0 | T | C | 95.7% | 144.7 / ‑2.2 | 46 | intergenic (+512/‑80) | ompC/hchA | porin OmpC/protein deglycase HchA |
| Reads supporting (aligned to +/- strand): ref base T (0/2); new base C (21/23); total (21/25) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.93e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
GCGTGAATAATATTCAACACCAGTGCAGCATCATCATTACATACTTACAGCCGCCGACTACCGCTTTCGATATATTGACTCAATTTTCCAGCCTGGTTAAAAATAGCGACCAGCTCAGTCGCAAATATAGTGACTACCCTAACTAAGCAACAATAAGGAATACACT > NZ_CP009273/2029150‑2029315 | gCGTGAATAATATTCAACACCAGTGCAGCATCATCATTACATACTTACAGCCGCCGACTACCGCTTTCGATATATTGACTCAATTTCCCa > 2:141293/1‑90 (MQ=255)gCGTGAATAATATTCAACACCAGTGCAGCATCATCATTACATACTTACAGCCGCCGACTACCGCTTTCGATATATTGACTCAATTTCCCa > 1:185530/1‑90 (MQ=255) gAATAATATTCAACACCAGTGCAGCATCATCATTACATACTTACAGCCGCCGACTACCGCTTTCGATATATTGACTCAATTTCCCAGCCt < 2:621626/90‑1 (MQ=255) cAACACCAGTGCAGCATCATCATTACATACTTACAGCCGCCGACTACCGCTTTCGATATATTGACTCAATTTCCCAGCCTGGTTAAAAAt > 2:753851/1‑90 (MQ=255) cAACACCAGTGCAGCATCATCATTACATACTTACAGCCGCCGACTACCGCTTTCGATATATTGACTCAATTTCCCAGCCTGGTTAAAAAt > 2:204092/1‑90 (MQ=255) cAACACCAGTGCAGCATCATCATTACATACTTACAGCCGCCGACTACCGCTTTCGATATATTGACTCAATTTCCCAGCCTGGTTAAAAAt > 1:27836/1‑90 (MQ=255) cAACACCAGTGCAGCATCATCATTACATACTTACAGCCGCCGACTACCGCTTTCGATATATTGACTCAATTTCCCAGCCTGGTTAAAAAt > 2:611552/1‑90 (MQ=255) aGTGCAGCATCATCATTACATACTTACAGCCGCCGACTACCGCTTTCGATATATTGACTCAATTTCCCAGCCTGGTTAAAAATAGCGAcc > 2:627226/1‑90 (MQ=255) aGTGCAGCATCATCATTACATACTTACAGCCGCCGACTACCGCTTTCGATATATTGACTCAATTTCCCAGCCTGGTTAAAAATAGCGAcc < 2:195629/90‑1 (MQ=255) aGTGCAGCATCATCATTACATACTTACAGCCGCCGACTACCGCTTTCGATATATTGACTCAATTTCCCAGCCGGGTTAAAAATAGCGAcc > 2:110086/1‑90 (MQ=255) gTGCAGCATCATCATTACATACTTACAGCCGCCGACTACCGCTTTCGATATATTGACTCAATTTCCCAGCCTGGTTAAAAATAGCGACCa > 1:167037/1‑90 (MQ=255) tcatcatTACATACTTACAGCCGCCGACTACCGCTTTCGATATATTGACTCAATTTCCCAGCCTGGTTAAAAATAGCGACCAGCTCAGTc < 2:149907/90‑1 (MQ=255) atcatTACATACTTACAGCCGCCGACTACCGCTTTCGATATATTGACTCAATTTCCCAGCCTGGTTAAAAATAGCGACCAGCTCAGTCGc > 2:580969/1‑90 (MQ=255) atcatTACATACTTACAGCCGCCGACTACCGCTTTCGATATATTGACTCAATTTCCCAGCCTGGTTAAAAATAGCGACCAGCTCAGTCGc > 1:383973/1‑90 (MQ=255) atcatTACATACTTACAGCCGCCGACTACCGCTTTCGATATATTGACTCAATTTCCCAGCCTGGTTAAAAATAGCGACCAGCTCAGTCGc < 1:627226/90‑1 (MQ=255) catTACATACTTACAGCCGCCGACTACCGCTTTCGATATATTGACTCAATTTCCCAGCCTGGTTAAAAATAGCGACCAGCTCAGTCGCaa < 1:634848/90‑1 (MQ=255) tACATACTTACAGCCGCCGACTACCGCTTTCGATATATTGACTCAATTTCCCAGCCTGGTTAAAAATAGCGACCAGCTCAGTCGCAAata > 2:105122/1‑90 (MQ=255) cATACTTACAGCCGCCGACTACCGCTTTCGATATATTGACTCAATTTCCCAGCCTGGTTAAAAATAGCGACCAGCTCAGTCGCAAAtata < 1:510554/90‑1 (MQ=255) cATACTTACAACCGCCGACTACCGCTTTCGATATATTGACTCAATTTCCCAGCCTGGTTAAAAATAGCGACCAGCTCAGTCGCAAAtata < 1:505112/90‑1 (MQ=255) aTACTTACAGCCGCCGACTACCGCTTTCGATATATTGACTCAATTTCCCAGCCTGGTTAAAAATAGCGACCAGCTCAGTCGCAAATATAg > 1:300905/1‑90 (MQ=255) cTTACAGCCGCCGACTACCGCTTTCGATATATTGACTCAATTTCCCAGCCTGGTTAAAAATAGCGACCAGCTCAGTCGCAAATATAGTGa < 2:300905/90‑1 (MQ=255) cTTACAGCCGCCGACTACCGCTTTCGATATATTGACTCAATTTCCCAGCCTGGTTAAAAATAGCGACCAGCTCAGTCGCAAATATAGTGa < 1:539473/90‑1 (MQ=255) cTTACAGCCGCCGACTACCGCTTTCGATATATTGACTCAATTTCCCAGCCTGGTTAAAAATAGCGACCAGCTCAGTCGCAAATATAGTGa < 1:355032/90‑1 (MQ=255) cTTACAGCCGCCGACTACCGCTTTCGATATATTGACTCAATTTCCCAGCCTGGTTAAAAATAGCGACCAGCTCAGTCGCAAATATAGTGa > 2:157668/1‑90 (MQ=255) ttACAGCCGCCGACTACCGCTTTCGATATATTGACTCAATTTCCCAGCCTGGTTAAAAATAGCGACCAGCTCAGTCGCAAATATAGTGAc < 2:257558/90‑1 (MQ=255) ttACAGCCGCCGACTACCGCTTTCGATATATTGACTCAATTTCCCAGCCTGGTTAAAAATAGCGACCAGCTCAGTCGCAAATATAGTGAc > 2:166615/1‑90 (MQ=255) aGCCGCCGACTACCGCTTTCGATATATTGACTCAATTTCCCAGCCTGGTTAAAAATAGCGACCAGCTCAGTCGCAAATATAGTGACTAcc > 1:622622/1‑90 (MQ=255) cgccgACTACCGCTTTCGATATATTGACTCAATTTCCCAGCCTGGTTAAAAATAGCGACCAGCTCAGTCGCAAATATAGTGACTACCcta > 1:765481/1‑90 (MQ=255) cgccgACTACCGCTTTCGATATATTGACTCAATTTCCCAGCCTGGTTAAAAATAGCGACCAGCTCAGTCGCAAATATAGTGACTACCcta > 1:131520/1‑90 (MQ=255) gccgACTACCGCTTTCGATATATTGACTCAATTTCCCAGCCTGGTTAAAAATAGCGACCAGCTCAGTCGCAAATATAGTGACTACCctaa > 2:528788/1‑90 (MQ=255) ccgACTACCGCTTTCGATATATTGACTCAATTTCCCAGCCTGGTTAAAAATAGCGACCAGCTCAGTCGCAAATATAGTGACTACCctaac < 1:675507/90‑1 (MQ=255) tACCGCTTTCGATATATTGACTCAATTTTCCAGCCTGGTTAAAAATAGCGACCAGCTCAGTCGCAAATATAGTGACTACCCTAACTAAGc < 1:379676/90‑1 (MQ=255) tACCGCTTTCGATATATTGACTCAATTTCCCAGCCTGGTTAAAAATAGCGACCAGCTCAGTCGCAAATATAGTGACTACCCTAACTAAGc < 2:44790/90‑1 (MQ=255) tACCGCTTTCGATATATTGACTCAATTTCCCAGCCTGGTTAAAAATAGCGACCAGCTCAGTCGCAAATATAGTGACTACCCTAACTAAGc < 1:250453/90‑1 (MQ=255) tACCGCTTTCGATATATTGACTCAATTTCCCAGCCTGGTTAAAAATAGCGACCAGCTCAGTCGCAAATATAGTGACTACCCTAACTAAGc < 1:611552/90‑1 (MQ=255) cGCTTTCGATATATTGACTCAATTTCCCAGCCTGGTTAAAAATAGCGACCAGCTCAGTCGCAAATATAGTGACTACCCTAACTAAGcaac < 2:27836/90‑1 (MQ=255) ttCGATATATTGACTCAATTTCCCAGCCTGGTTAAAAATAGCGACCAGCTCAGTCGCAAATATAGTGACTACCCTAACTAAGCAACAATa < 2:383973/90‑1 (MQ=255) ttCGATATATTGACTCAATTTCCCAGCCTGGTTAAAAATAGCGACCAGCTCAGTCGCAAATATAGTGACTACCCTAACTAAGCAACAATa < 1:186651/90‑1 (MQ=255) ttCGATATATTGACTCAATTTCCCAGCCTGGTTAAAAATAGCGACCAGCTCAGTCGCAAATATAGTGACTACCCTAACTAAGCAACAATa < 1:166615/90‑1 (MQ=255) tatatTGACTCAA‑TTTCCAGCCTGGTTAAAAATAGCGACCAGCTCAGTCGCAAATATAGTGACTACCCTAACTAAGCAACAATAAGGAAt < 1:553718/90‑1 (MQ=255) atatTGACTCAATTTCCCAGCCTGGTTAAAAATAGCGACCAGCTCAGTCGCAAATATAGTGACTACCCTAACTAAGCAACAATAAGGAAt < 2:131520/90‑1 (MQ=255) atatTGACTCAATTTCCCAGCCTGGTTAAAAATAGCGACCAGCTCAGTCGCAAATATAGTGACTACCCTAACTAAGCAACAATAAGGAAt < 1:576282/90‑1 (MQ=255) atatTGACTCAATTTCCCAGCCTGGTTAAAAATAGCGACCAGCTCAGTCGCAAATATAGTGACTACCCTAACTAAGCAACAATAAGGAAt < 1:204092/90‑1 (MQ=255) gACTCAATTTCCCAGCCTGGTTAAAAATAGCGACCAGCTCAGTCGCAAATATAGTGACTACCCTAACTAAGCAACAATAAGGAATACACt > 2:567825/1‑90 (MQ=255) aaTTTCCCAGCCTGGTTAAAAATAGCGACCAGCTCAGTCGCAAATATAGTGACTACCCTAACTAAGCAACAATAAGGAAt < 1:473960/80‑1 (MQ=255) aaTTTCCCAGCCTGGTTAAAAATAGCGACCAGCTCAGTCGCAAATATAGTGACTACCCTAACTAAGCAACAATAAGGAAt > 2:473960/1‑80 (MQ=255) | GCGTGAATAATATTCAACACCAGTGCAGCATCATCATTACATACTTACAGCCGCCGACTACCGCTTTCGATATATTGACTCAATTTTCCAGCCTGGTTAAAAATAGCGACCAGCTCAGTCGCAAATATAGTGACTACCCTAACTAAGCAACAATAAGGAATACACT > NZ_CP009273/2029150‑2029315 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |