Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	231,042	A→C	G76G (GGT→GGG)	gloB ←	hydroxyacylglutathione hydrolase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	231,042	0	A	C	100.0%	90.0 / NA	26	G76G (GGT→GGG)	gloB	hydroxyacylglutathione hydrolase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (15/11);  total (15/11)

TACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGACCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCGTGGTGATGGTGGGTGAGAAAT  >  NZ_CP009273/230958‑231123                                                                                     |                                                                                  tACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATa                                                                              >  2:93340/1‑90 (MQ=255) tACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATa                                                                              <  1:417064/90‑1 (MQ=255) tACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATa                                                                              >  1:718651/1‑90 (MQ=255)             aTGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTtg                                                                  >  2:579773/1‑90 (MQ=255)              tGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTtgt                                                                 >  2:10349/1‑90 (MQ=255)                   cAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGaaa                                                            >  1:464018/1‑90 (MQ=255)                             gCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTccacc                                                  <  2:216152/90‑1 (MQ=255)                                        aTCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTAc                                       <  1:79838/90‑1 (MQ=255)                                                  aCCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACg                             <  1:637712/90‑1 (MQ=255)                                                  aCCTGTGTTGTTCCCTTATCTTGTGTCTCTTGGGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACg                             >  1:168542/1‑90 (MQ=255)                                                   ccTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGt                            <  1:647792/90‑1 (MQ=255)                                                      gtgtTGTTCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGAt                         >  2:763869/1‑90 (MQ=255)                                                       tgttgtTCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATc                        <  1:763869/90‑1 (MQ=255)                                                       tgttgtTCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATc                        <  1:239656/90‑1 (MQ=255)                                                        gttgttCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCg                       >  1:10884/1‑90 (MQ=255)                                                             tCCCTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCgtggtg                  >  2:397018/1‑90 (MQ=255)                                                                cTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTccacca                                                 >  1:99748/1‑56 (MQ=255)                                                                cTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTccacca                                                 <  2:99748/56‑1 (MQ=255)                                                                cTTATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCGTGGTGAtg               >  1:571609/1‑90 (MQ=255)                                                                  tATCTTGTGTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCGTGGTGAtggt             <  2:108388/90‑1 (MQ=255)                                                                       tgtgTCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCGTGGTGATggtgggtg        <  2:481757/90‑1 (MQ=255)                                                                        gtgtCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCGTGGTGATGGTGGGTga       >  1:442742/1‑90 (MQ=255)                                                                        gtgtCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCGTGGTGATGGTGGGTga       >  2:41547/1‑90 (MQ=255)                                                                        gtgtCTCTTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCGTGGTGATGGTGGGTga       <  2:571609/90‑1 (MQ=255)                                                                            ctctTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCGTGGTGATGGTGGGTGAGaaa   >  1:688204/1‑90 (MQ=255)                                                                             tctTGTGGCCCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCGTGGTGATGGTGGGTGATAAat  >  1:221613/1‑90 (MQ=255)                                                                                     |                                                                                  TACACTAAATTCATGCCCCAAAACGAAGGCAGTTTCGCCATCTTTGACTACCTGTGTTGTTCCCTTATCTTGTGTCTCTTGTGGACCATACACCACAATTTGTGGAAACTTTTCCACCAGTTCTTTTACGCCGCCAACGTGATCGTGGTGATGGTGGGTGAGAAAT  >  NZ_CP009273/230958‑231123

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑