Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 2,189,312 A→G T512A (ACC→GCC)  metG → methionine‑‑tRNA ligase

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,189,3120AG100.0% 15.5 / NA 6T512A (ACC→GCC) metGmethionine‑‑tRNA ligase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (3/3);  total (3/3)

ACCTGTTCCGCGTGCTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGC  >  NZ_CP009273/2189226‑2189344
                                                                                      |                                
aCCTGTTCCGCGTGCTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGGCCt                               <  1:278851/90‑1 (MQ=255)
  cTGTTCCGCGTGCTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGGCCTgg                             >  1:414938/1‑90 (MQ=255)
     ttCCGCGTGCTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGGCCTGGGAt                          <  2:106560/90‑1 (MQ=255)
                gatgaCTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGGCCTGGGATGGTATCCAGCa               >  2:202443/1‑90 (MQ=255)
                   gaCTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGGGCAGAAGCATTCCTCAATCCGGAACTGGCCTGGGATGGTATCCAGCAAcc            <  1:772391/90‑1 (MQ=255)
                             aaGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGGCCTGGGATGGTATCCAGCAACCGCTGCTGGGc  >  1:443722/1‑90 (MQ=255)
                                                                                      |                                
ACCTGTTCCGCGTGCTGATGACTTACCTGAAGCCGGTACTGCCGAAACTGACCGAGCGTGCAGAAGCATTCCTCAATACGGAACTGACCTGGGATGGTATCCAGCAACCGCTGCTGGGC  >  NZ_CP009273/2189226‑2189344

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: