Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	232,840	T→A	V96E (GTG→GAG)	dnaQ →	DNA polymerase III subunit epsilon

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	232,840	0	T	A	100.0%	66.1 / NA	18	V96E (GTG→GAG)	dnaQ	DNA polymerase III subunit epsilon
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (3/15);  total (3/15)

GTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTT  >  NZ_CP009273/232753‑232924                                                                                        |                                                                                     gTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGgaga                                                                                    <  1:154977/90‑1 (MQ=255) gTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGgaga                                                                                    <  2:686574/90‑1 (MQ=255) gTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGgaga                                                                                    <  1:490333/90‑1 (MQ=255) gTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGgaga                                                                                    <  2:318274/90‑1 (MQ=255) gTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGgaga                                                                                    <  1:613122/90‑1 (MQ=255) gTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGgaga                                                                                    <  2:299510/90‑1 (MQ=255) gTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGgaga                                                                                    <  2:185452/90‑1 (MQ=255)       ccGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGAGATCCATa                                                                              >  2:254065/1‑90 (MQ=255)        cGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGAGATCCATaa                                                                             >  1:552673/1‑90 (MQ=255)           tGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGAGATCCATAACGc                                                                          <  2:363497/90‑1 (MQ=255)             aaTTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGAGATCCATAACGCAg                                                                        <  2:725843/90‑1 (MQ=255)              aTTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGAGATCCATAACGCAGc                                                                       <  1:116432/90‑1 (MQ=255)              aTTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGAGATCCATAACGCAGc                                                                       <  2:82133/90‑1 (MQ=255)                 tttGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGAGATCCATAACGCAGCGtt                                                                    <  1:133939/90‑1 (MQ=255)                        gATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGAGATCCATAACGCAGCGTTCGATATc                                                             <  1:446310/90‑1 (MQ=255)                                cACTTTTGCCGATGTAGCCGATGGGTTCATGGACTATATTCGCGGCGCGGAGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTAt                                                     <  1:426431/90‑1 (MQ=255)                                cACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTAt                                                     <  2:552673/90‑1 (MQ=255)                                 aCGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTATg                                                    >  2:600891/1‑90 (MQ=255)                                  cGTTTGCCGAAGTAGCCGAAGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTATgg                                                   <  2:751059/90‑1 (MQ=255)                                     ttGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACt                                                <  1:691013/90‑1 (MQ=255)                                                         ttCATGGACTATATTCGCGGCGCGGAGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAg                            <  1:254065/90‑1 (MQ=255)                                                               gACTATATTCGCGGCGCGGAGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGat                      <  2:275235/90‑1 (MQ=255)                                                                          cggcgcggAGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGAc           <  1:536468/90‑1 (MQ=255)                                                                                 gAGTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACt    <  1:668882/90‑1 (MQ=255)                                                                                   gTTGGAGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACttt  <  1:600891/90‑1 (MQ=255)                                                                                        |                                                                                     GTATTGCCGATGAATTTTTGCTCGATAAGCCCACGTTTGCCGAAGTAGCCGATGAGTTCATGGACTATATTCGCGGCGCGGAGTTGGTGATCCATAACGCAGCGTTCGATATCGGCTTTATGGACTACGAGTTTTCGTTGCTTAAGCGCGATATTCCGAAGACCAATACTTT  >  NZ_CP009273/232753‑232924

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑