Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,396,099	G→C	A413G (GCC→GGC)	nuoC ←	NADH‑quinone oxidoreductase subunit C/D

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,396,099	0	G	C	100.0%	69.7 / NA	21	A413G (GCC→GGC)	nuoC	NADH‑quinone oxidoreductase subunit C/D
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (11/10);  total (11/10)

GCGCCTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGACGCCAGA  >  NZ_CP009273/2396013‑2396167                                                                                       |                                                                     gcgcCTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAgccgg                                                                   >  1:100012/1‑90 (MQ=255) gcgcCTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAgccgg                                                                   >  2:655269/1‑90 (MQ=255) gcgcCTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAgccgg                                                                   >  2:325126/1‑90 (MQ=255)     cTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAAc                                                               <  1:33715/90‑1 (MQ=255)     cTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAAc                                                               <  2:411402/90‑1 (MQ=255)              gTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGa                                                      <  1:734616/90‑1 (MQ=255)              gTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGa                                                      >  2:734616/1‑90 (MQ=255)                cGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAAc                                                    <  1:655269/90‑1 (MQ=255)                 gAAGTCGATCCCGGTAGCACGCACGCCCGCGCCAGGGGTGCCCCACTCCAGCGCCCCTTTCGCCCCATAGCCGGCAACGCCCTGGGAACg                                                   >  1:40964/1‑90 (MQ=255)                  aaGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGa                                                  >  2:329800/1‑90 (MQ=255)                   aGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGCGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGAc                                                 <  2:748448/90‑1 (MQ=255)                        aTCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTc                                            <  2:626802/90‑1 (MQ=255)                                 gCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTCAGAATGgtg                                   >  2:715722/1‑90 (MQ=255)                                   aCGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGCCCTTTCAGAATGGGGtt                                 >  2:658335/1‑90 (MQ=255)                                      cAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTATTg                              >  1:223227/1‑90 (MQ=255)                                            cgcgCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAgcgc                        <  2:523567/90‑1 (MQ=255)                                             gcgcCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTCAGAATGGGGTTTTGCAGcgcc                       >  1:582669/1‑90 (MQ=255)                                              cgcCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGcgccg                      >  2:109743/1‑90 (MQ=255)                                                 cAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCtt                   <  2:328328/90‑1 (MQ=255)                                                                  aGCGCCTCTTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGACGCCAGa  >  1:171619/1‑90 (MQ=255)                                                                        tctTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTc                                            >  1:774128/1‑42 (MQ=255)                                                                        tctTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTc                                            <  2:774128/42‑1 (MQ=255)                                                                        tctTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTCAGAAt                                       >  2:424684/1‑47 (MQ=255)                                                                        tctTTCGCGCCATAGCCGGCAACGCCCTGGGAACGACCTTTCAGAAt                                       <  1:424684/47‑1 (MQ=255)                                                                                       |                                                                     GCGCCTTACGCACGTCGAAGTCGATCCCGGTAGCACGCAGGCCCGCGCCAGTGGTGCCCCACTCCAGCGCCTCTTTCGCGCCATAGGCGGCAACGCCCTGGGAACGACCTTTCAGAATGGTGTTTTGCAGCGCCGCTTTCTCGTAAGACGCCAGA  >  NZ_CP009273/2396013‑2396167

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 11 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑