Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,425,778	T→C	E215G (GAA→GGA)	folC ←	bifunctional tetrahydrofolate synthase/dihydrofolate synthase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,425,778	0	T	C	100.0%	75.3 / NA	22	E215G (GAA→GGA)	folC	bifunctional tetrahydrofolate synthase/dihydrofolate synthase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (15/7);  total (15/7)

GTGCCGTGAGCATCGCTAAACGCCCAGTCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGCTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCG  >  NZ_CP009273/2425691‑2425864                                                                                        |                                                                                       gTGCCGTGACCGTCGATGAACGCGCAGTCATGAGCGGTGGCGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCGCGTTTTccc                                                                                      <  1:429412/90‑1 (MQ=255)       tGAGCATCGCTAAACGCCCAGTCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTCCCTGCGcc                                                                                <  1:336668/90‑1 (MQ=255)                  aaaCGCCCAGTCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTCCCTGCGCCACATCAGCAAt                                                                     <  1:432386/90‑1 (MQ=255)                       cccAGTCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTCCCTGCGCCACATCAGCAATGGTAg                                                                <  2:462392/90‑1 (MQ=255)                                     gTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTCCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGc                                                  >  1:127673/1‑90 (MQ=255)                                     gTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTCCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGc                                                  <  2:488985/90‑1 (MQ=255)                                     gTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTCCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGc                                                  >  1:523044/1‑90 (MQ=255)                                     gTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTCCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGc                                                  >  1:293078/1‑90 (MQ=255)                                     gTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTCCCTGCGCCACATCAGCAATGGGAGAAGGCATTTCCGGc                                                  >  1:84446/1‑90 (MQ=255)                                                     aCTCAACGCCCCGACGTTGTAACAGTGCACCTTTTCCCTGCGCCACATCAGCAAt                                                                     >  1:469701/1‑55 (MQ=255)                                                     aCTCAACGCCCCGACGTTGTAACAGTGCACCTTTTCCCTGCGCCACATCAGCAAt                                                                     <  2:469701/55‑1 (MQ=255)                                                     aCTCAACGCCCCGACGTTGTAACAGTGCACCTTTTCCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGCTCACCg                                            >  1:24899/1‑80 (MQ=255)                                                     aCTCAACGCCCCGACGTTGTAACAGTGCACCTTTTCCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGCTCACCg                                            <  2:24899/80‑1 (MQ=255)                                                          aCGCCCCGACGTTGTAACAGTGCACCTTTTCCCTGCGcc                                                                                <  1:27995/39‑1 (MQ=39)                                                          aCGCCCCGACGTTGTAACAGTGCACCTTTTCCCTGCGcc                                                                                >  2:27995/1‑39 (MQ=39)                                                             ccccGACGTTGTAACAGTGCACCTTTTCCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGCTCACCGACAATTGCCGGTTTTTCg                          >  2:52477/1‑90 (MQ=255)                                                                cGACGTTGTAACAGTGCACCTTTTCCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGCTCACCGACAATTGCCGGTTTTTCGCTg                       >  2:418186/1‑90 (MQ=255)                                                                            aGTGCACCTTTTCCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGCTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGCCt           >  2:583282/1‑90 (MQ=255)                                                                             gTGCACCTTTTCCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGCTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTg          >  1:184609/1‑90 (MQ=255)                                                                             gTGCACCTTTTCCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGCTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTg          >  1:248008/1‑90 (MQ=255)                                                                              tGCACCTTTTCCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGCTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGc         >  2:42299/1‑90 (MQ=255)                                                                              tGCACCTTTTCCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGCTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGc         >  2:675820/1‑90 (MQ=255)                                                                               gCACCTTTTCCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGCTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCt        >  1:762929/1‑90 (MQ=255)                                                                                     tttcCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGCTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTcg  <  2:392026/90‑1 (MQ=255)                                                                                     tttcCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGCTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTcg  <  2:682289/90‑1 (MQ=255)                                                                                        |                                                                                       GTGCCGTGAGCATCGCTAAACGCCCAGTCATGATCGGTGACGGAATAGTTCCACTCAACGCCCCGACGTTGTAACAGTGCACCTTTTTCCTGCGCCACATCAGCAATGGTAGAAGGCATTTCCGGCTCACCGACAATTGCCGGTTTTTCGCTGCGGAAGATGCCTGCTTTCTCG  >  NZ_CP009273/2425691‑2425864

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑