Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,434,529	A→C	F186V (TTT→GTT)	fabB ←	beta‑ketoacyl‑ACP synthase I

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,434,529	0	A	C	100.0%	128.8 / NA	36	F186V (TTT→GTT)	fabB	beta‑ketoacyl‑ACP synthase I
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (15/21);  total (15/21)

TGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGTTACCGATACAGTGTGCGGAAGTCGCACACGCGGAGCTGATGGAG  >  NZ_CP009273/2434445‑2434614                                                                                     |                                                                                      tgtCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGa                                                                                  <  2:77907/90‑1 (MQ=255)          aTTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGtt                                                                         >  1:107715/1‑90 (MQ=255)             tagtagACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGc                                                                      <  2:9631/90‑1 (MQ=255)                      gcgcACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGa                                                             >  1:119919/1‑90 (MQ=255)                      gcgcACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGa                                                             >  2:583469/1‑90 (MQ=255)                      gcgcACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGa                                                             >  2:435260/1‑90 (MQ=255)                      gcgcACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGa                                                             >  1:505579/1‑90 (MQ=255)                       cgcACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGcc                                                                     <  1:60471/81‑1 (MQ=255)                       cgcACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGcc                                                                     >  2:60471/1‑81 (MQ=255)                       cgcACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGACAGCAACCACGATGTCCTGTTTGCCCAGTTGGAt                                                            >  2:579911/1‑90 (MQ=255)                        gcACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATc                                                           <  2:107715/90‑1 (MQ=255)                        gcACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATc                                                           <  2:705478/90‑1 (MQ=255)                           cccATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGc                                                        <  2:401800/90‑1 (MQ=255)                              aTTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGctct                                                     >  2:454617/1‑90 (MQ=255)                               ttGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTa                                                    >  2:186044/1‑90 (MQ=255)                               ttGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTa                                                    <  2:671056/90‑1 (MQ=255)                                tGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCCGTTGGATCTGCTCTAc                                                   <  1:403036/90‑1 (MQ=255)                                tGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTAc                                                   <  2:759495/90‑1 (MQ=255)                                tGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTAc                                                   <  2:505579/90‑1 (MQ=255)                                 gCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACt                                                  >  1:560973/1‑90 (MQ=255)                                    tCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCg                                               <  2:560973/90‑1 (MQ=255)                                      gAATTCGCAAGCCATTTCCCAGCACAGCTCTTCTCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGtt                                             <  1:653555/90‑1 (MQ=255)                                      gAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGtt                                             <  2:312276/90‑1 (MQ=255)                                      gAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGtt                                             <  2:717930/90‑1 (MQ=255)                                                    tttCCCAGCACAGCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGTTACCGATACAGtgtg                               >  1:353220/1‑90 (MQ=255)                                                                gCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCg                                               <  2:120639/62‑1 (MQ=255)                                                                gCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCg                                               >  1:120639/1‑62 (MQ=255)                                                                gCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGtt                                             <  1:53596/64‑1 (MQ=255)                                                                gCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGtt                                             >  2:53596/1‑64 (MQ=255)                                                                gCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGTTACCGATACAg                                   <  2:714371/74‑1 (MQ=255)                                                                gCTCTTCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGTTACCGATACAg                                   >  1:714371/1‑74 (MQ=255)                                                                     tCGCCGCCGCCAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGTTACCGATACAGTGTGCGGAAGTCGCACAcgcg              >  1:439241/1‑90 (MQ=255)                                                                          gccgccAGCAACCACGATGTCCTGTTTGCCCAGTTGGTTCTGCTCTACTGCGTTACCGATACAGTGTGCGGAAGTCGCACACGCGGAGCt         <  2:439241/90‑1 (MQ=255)                                                                          gccgccAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGTTACCGATACAGTGTGCGGAAGTCGCACACGCGGAGCt         <  2:437359/90‑1 (MQ=255)                                                                             gccAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGTTACCGATACAGTGTGCGGAAGTCGCACACGCGGAGCTGAt      <  1:435260/90‑1 (MQ=255)                                                                              ccAGCAACCACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGTT‑‑‑GATACAGTGTGCGGAAGTCGCACACGCGGAGCTGATGGag  <  1:583469/90‑1 (MQ=255)                                                                                     |                                                                                      TGTCGTTGTATTTAGTAGACAGCGCACCCATTGCGTCGAATTCGCAAGCCATTTCCCAGCACAGCTCTTCGCCGCCGCCAGCAAACACGATGTCCTGTTTGCCCAGTTGGATCTGCTCTACTGCGTTACCGATACAGTGTGCGGAAGTCGCACACGCGGAGCTGATGGAG  >  NZ_CP009273/2434445‑2434614

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑