Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 2,505,777 T→C I137V (ATC→GTC)  mntH ← Nramp family divalent metal transporter

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,505,7770TC100.0% 44.3 / NA 14I137V (ATC→GTC) mntHNramp family divalent metal transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/4);  total (10/4)

CGGCAACAAACAACAGTAACCCGCCAATCACTTTCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTC  >  NZ_CP009273/2505690‑2505861
                                                                                       |                                                                                    
cgGCAACAAACAACAGTAACCCGCCAATCACTTTCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGAccc                                                                                    >  2:315259/1‑90 (MQ=255)
cgGCAACAAACAACAGTAACCCGCCAATCACTTTCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGAccc                                                                                    <  2:714432/90‑1 (MQ=255)
          caacaGTAACCCGCCAATCACTTTCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGACCCCCGTCAGCAc                                                                          >  2:626514/1‑90 (MQ=255)
                      gCCAATCACTTTCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTTGCGACCCCCGTCAGCACCGCGCCCTGcaa                                                              >  2:513439/1‑90 (MQ=255)
                      gCCAATCACTTTCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGACCCCCGTCAGCACCGCGCCCTGcaa                                                              >  1:10220/1‑90 (MQ=255)
                      gCCAATCACTTTCTCCAGCGATTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGACCCCCGTCAGCACCGCGCCCTGcaa                                                              >  1:149464/1‑90 (MQ=255)
                                 tctcCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGACCCCCGTCAGCACCGCGCCCTGCAACAACGAAacac                                                   <  2:597825/90‑1 (MQ=255)
                                       gCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGACCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGaa                                             >  2:92650/1‑90 (MQ=255)
                                         ggTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGACCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATg                                           <  2:177267/90‑1 (MQ=255)
                                          gTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGACCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGa                                          <  1:369371/90‑1 (MQ=255)
                                              ttGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGACCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGttt                                      >  2:421906/1‑90 (MQ=255)
                                                    gcgACGTTGCAGCATTAAAATCAGGAAAGTCGCGACCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAAcc                                >  1:622175/1‑90 (MQ=255)
                                                    gcgACGTTGCAGCATTAAAATCAGGAAAGTCGCGACCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAAcc                                >  2:482250/1‑90 (MQ=255)
                                                               gcaTTAAAATCAGGAAAGTCGCGACCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCAc                     <  2:53656/90‑1 (MQ=255)
                                                                         cAGGAAAGTCGCGACCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTc           >  1:198837/1‑90 (MQ=255)
                                                                                  cgcgACCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTc  >  1:488758/1‑90 (MQ=255)
                                                                                       |                                                                                    
CGGCAACAAACAACAGTAACCCGCCAATCACTTTCTCCAGCGGTTTTTGCCCGCGACGTTGCAGCATTAAAATCAGGAAAGTCGCGATCCCCGTCAGCACCGCGCCCTGCAACAACGAAACACCAAGAATGAGTTTAAAACCGATCGCCGCACCAATAAATTCCGCCAGGTC  >  NZ_CP009273/2505690‑2505861

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: