Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,591,366	C→T	D287N (GAC→AAC)	bamC ←	outer membrane protein assembly factor BamC

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,591,366	0	C	T	100.0%	85.5 / NA	23	D287N (GAC→AAC)	bamC	outer membrane protein assembly factor BamC
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base T (15/8);  total (15/8)

GCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTT  >  NZ_CP009273/2591283‑2591432                                                                                    |                                                                   gctgctGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCACGCCTGGGTCGCCCGCGCCCAGGTCCTGCCAGTTGCTGTc                                                              >  1:371348/1‑90 (MQ=255)    gcGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTTGCTGTCAGa                                                           >  1:77691/1‑90 (MQ=255)        ttATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTTGCTGTCAGACAGc                                                       <  2:221617/90‑1 (MQ=255)        ttATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTTGCTGTCAGACAGc                                                       <  1:632954/90‑1 (MQ=255)         tATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTTGCTGTCAGACAGCg                                                      <  1:706261/90‑1 (MQ=255)               aaTCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTTGCTGTCAGACAGCGGCTtat                                                <  1:457304/90‑1 (MQ=255)                 tCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTTGCTGTCAGACAGCGGCTTATaa                                              >  1:98093/1‑90 (MQ=255)                   gCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTTGCTGTCAGACAGCGGCTTATAAGt                                            >  1:503692/1‑90 (MQ=255)                    ccAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTTGCTGTCAGACAGCGGCTTATAAGtt                                           >  1:603631/1‑90 (MQ=255)                           gCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTTGCTGTCAGACAGCGGCTTATAAGTTACGGCCa                                    >  1:416549/1‑90 (MQ=255)                                      tCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTTGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGccc                            <  2:350776/87‑1 (MQ=255)                                      tCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTTGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGccc                            >  1:350776/1‑87 (MQ=255)                                        aCCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTTGCTGTCAGACAGc                                                       >  2:539172/1‑58 (MQ=255)                                        aCCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTTGCTGTCAGACAGc                                                       <  1:539172/58‑1 (MQ=255)                                        aCCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTTGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGa                       >  1:593114/1‑90 (MQ=255)                                        aCCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTTGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGa                       >  2:262004/1‑90 (MQ=255)                                            gATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTTGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACgg                   >  1:294704/1‑90 (MQ=255)                                                   ggCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTTGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGt            <  2:294704/90‑1 (MQ=255)                                                        ggATCGCTCGCGCCCAGTTCCTGCCAGTTGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTc       <  2:528534/90‑1 (MQ=255)                                                             gctcgcGCCCAGTTCCTGCCAGTTGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACttt  >  1:81062/1‑90 (MQ=255)                                                             gctcgcGCCCAGTTCCTGCCAGTTGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACttt  >  2:176654/1‑90 (MQ=255)                                                             gctcgcGCCCAGTTCCTGCCAGTTGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACttt  >  1:296567/1‑90 (MQ=255)                                                             gctcgcGCCCAGTTCCTGCCAGTTGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACttt  >  2:433723/1‑90 (MQ=255)                                                                                    |                                                                   GCTGCGGTTATCTAAATCGCCAACCTGCAGTTTATAGTCACCGGATGCCAGGCCTGGATCGCTCGCGCCCAGTTCCTGCCAGTCGCTGTCAGACAGCGGCTTATAAGTTACGGCCATGTTGCCCTGCGAACGGGTGCTGTCGGTCACTTT  >  NZ_CP009273/2591283‑2591432

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑