Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,670,359	T→C	G454G (GGA→GGG)	yphE ←	sugar ABC transporter ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,670,359	0	T	C	100.0%	130.9 / NA	37	G454G (GGA→GGG)	yphE	sugar ABC transporter ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (21/16);  total (21/16)

CGTGCTGTAATAACAGGATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGACGCCGCGCGTTGGCTCGTCGAGC  >  NZ_CP009273/2670283‑2670440                                                                             |                                                                                  cgtgcTGTAATAACAGGATGCGGTCACACCCCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGcagcca                                                                      <  1:173028/90‑1 (MQ=255)    gcTGTAATAACAGGATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCt                                                                   >  1:739680/1‑90 (MQ=255)      tGTAATAACAGGATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCa                                                                 <  1:642509/90‑1 (MQ=255)         aataaCAGGATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACgg                                                              <  1:89622/90‑1 (MQ=255)         aataaCAGGATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACgg                                                              >  2:36725/1‑90 (MQ=255)         aataaCAGGATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACgg                                                              <  2:559663/90‑1 (MQ=255)         aataaCAGGATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACgg                                                              <  1:557583/90‑1 (MQ=255)         aataaCAGGATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGAAGCCAGCTCACgg                                                              >  1:546839/1‑90 (MQ=255)                 gATGCGGTCACACACCAGCGGCAACTCCTCCGCTTAAGTGAAGATAAACACCACGCTTTTCCCTTCGGCAGACAGCTCACGGACAAttca                                                      >  1:238512/1‑87 (MQ=255)                 gATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGcagcca                                                                      >  1:485001/1‑74 (MQ=255)                 gATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGcagcca                                                                      <  2:485001/74‑1 (MQ=255)                 gATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACgg                                                              <  1:508650/82‑1 (MQ=255)                 gATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACgg                                                              >  2:508650/1‑82 (MQ=255)                 gATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACg                                                      >  1:277518/1‑90 (MQ=255)                      ggTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTaaa                                                 >  1:57887/1‑90 (MQ=255)                      ggTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTaaa                                                 <  2:550711/90‑1 (MQ=255)                         cacacaCCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATct                                              <  2:277518/90‑1 (MQ=255)                                cAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGttt                                       <  2:496890/90‑1 (MQ=255)                                       aaCTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGcagcca                                                                      >  1:686228/1‑52 (MQ=255)                                       aaCTCCTCCACTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGcagcca                                                                      <  2:686228/52‑1 (MQ=255)                                                 cTTCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGAcgc                      >  2:667125/1‑90 (MQ=255)                                                   tCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACgg                                                              >  1:187266/1‑48 (MQ=255)                                                   tCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACgg                                                              <  2:328520/48‑1 (MQ=255)                                                   tCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACgg                                                              <  2:187266/48‑1 (MQ=255)                                                   tCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACgg                                                              >  1:328520/1‑48 (MQ=255)                                                   tCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATctgc                                            <  1:661163/66‑1 (MQ=255)                                                   tCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATctgc                                            >  2:661163/1‑66 (MQ=255)                                                   tCACTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGAcgccg                    >  2:63603/1‑90 (MQ=255)                                                     aCTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGACGCcgcg                  >  1:45192/1‑90 (MQ=255)                                                      cTGGAGATAAACACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGACGCcgcgc                 >  1:768454/1‑90 (MQ=255)                                                        ggAGATAAACACCACGCTTTTCCCTTCGGcagcca                                                                      >  2:22538/1‑35 (MQ=38)                                                        ggAGATAAACACCACGCTTTTCCCTTCGGcagcca                                                                      <  1:22538/35‑1 (MQ=38)                                                              aaaCACCACGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGACGCCGCGCGTTGGCtc         <  1:702958/90‑1 (MQ=255)                                                                  accacGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGACGCCGCGCGTTGGCtcgtcg     >  1:643392/1‑90 (MQ=255)                                                                   ccacGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGACGCCGCGCGTTGGCTCGTCGa    >  2:397914/1‑90 (MQ=255)                                                                    cacGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGACGCCGCGCGTTGGCTCGTCGAg   >  2:424675/1‑90 (MQ=255)                                                                     acGCTTTTCCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCCTCGATATCGACGCCGCGCGTTGGCTCGTCGAGc  >  1:753311/1‑90 (MQ=255)                                                                             |                                                                                  CGTGCTGTAATAACAGGATGCGGTCACACACCAGCGGCAACTCCTCCACTTCACTGGAGATAAACACCACGCTTTTTCCTTCGGCAGCCAGCTCACGGACAATACGGTAAATCTGCTGTTTGGCTTCGATATCGACGCCGCGCGTTGGCTCGTCGAGC  >  NZ_CP009273/2670283‑2670440

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑