Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,678,723	A→C	G48G (GGT→GGG)	glyA ←	serine hydroxymethyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,678,723	0	A	C	100.0%	37.4 / NA	12	G48G (GGT→GGG)	glyA	serine hydroxymethyltransferase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (6/6);  total (6/6)

AGTTGTTCAACGATATCAACATACTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGAACCCTGCGCCTGCATTACGCGCGGGCTGGTGTAGTTTTCGGAGGCGATCAGTTCGATGTGCTCTTCCTGACGTACTT  >  NZ_CP009273/2678637‑2678799                                                                                       |                                                                             aGTTGTTCAACGATATCAACATACTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGAcccc                                                                           >  2:93292/1‑90 (MQ=255)  tttgtaCAACAATATCACCATCCTCCCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGACCCCt                                                                          <  1:427209/89‑1 (MQ=255)        aaaCGATATAAACATCCCCGAAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGACCCCTGCGCCt                                                                    <  2:179516/89‑1 (MQ=255)            gATATCAACATACTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGACCCCTGCGCCTGCAt                                                                >  1:168285/1‑90 (MQ=255)              tacaaaCATCCCCCCACCCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGACCCCTGCGCCTGCATTa                                                              <  1:663482/86‑1 (MQ=255)                   aCATCCCCGCACCCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGACCCCTGCGCCTGCATTAcgcgc                                                         <  1:396831/90‑1 (MQ=255)                                  ccgTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGACCCCTGCGCCTGCATTACGCGCGGGCTGGTGTAGttt                                          >  2:721271/1‑90 (MQ=255)                                                    ggATAACCTTCAGCATATTTGTTGGTAAGCTGAGACCCCTGCGCCGGCATTACGCGCGGGCTGGTGTAGTTTTCGGAGGCGATCAGTTCg                        >  1:188924/1‑90 (MQ=255)                                                                    atTTGTTGGTCAGCTGAGACCCCTGCGCCTGCATTACGCGCGGGCTGGTGTAGTTTTCGGAGGCGATCAGTTCGATGTGc                  >  1:296702/1‑80 (MQ=255)                                                                    atTTGTTGGTCAGCTGAGACCCCTGCGCCTGCATTACGCGCGGGCTGGTGTAGTTTTCGGAGGCGATCAGTTCGATGTGc                  <  2:296702/80‑1 (MQ=255)                                                                    atTTGTTGGTCAGCTGAGACCCCTGCGCCTGCATTACGCGCGGGCTGGTGTAGTTTTCGGAGGCGATCAGTTCGATGTGCTCTTCCTGAc        >  1:473731/1‑90 (MQ=255)                                                                     tttGTTGGTCAGCTGAGACCCCTGCGCCTGCATTACGCGCGGGCTGGTGTAGTTTTCGGAGGCGATCAGTTCGATGTGCTCTTCCTGACg       <  1:721271/90‑1 (MQ=255)                                                                          tgGTCAGCTGAGACCCCTGCGCCTGCATTACGCGCGGGCTGGTGTAGTTTTCGGAGGCGATCAGTTCGATGTGCTCTTCCTGACGTACtt  >  2:224262/1‑90 (MQ=255)                                                                                       |                                                                             AGTTGTTCAACGATATCAACATACTCGCAACCGCCGTAGTAGCGTTTGCCCGGATAACCTTCAGCATATTTGTTGGTCAGCTGAGAACCCTGCGCCTGCATTACGCGCGGGCTGGTGTAGTTTTCGGAGGCGATCAGTTCGATGTGCTCTTCCTGACGTACTT  >  NZ_CP009273/2678637‑2678799

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑