Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	297,509	G→C	G30G (GGC→GGG)	paoB ←	aldehyde oxidoreductase FAD‑binding subunit PaoB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	297,509	0	G	C	100.0%	88.8 / NA	26	G30G (GGC→GGG)	paoB	aldehyde oxidoreductase FAD‑binding subunit PaoB
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (11/15);  total (11/15)

ATCTTATCGAGCCCGAGGCCGTTCACATCGATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCGCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAA  >  NZ_CP009273/297423‑297589                                                                                       |                                                                                 aTCTTATCGAGCCCGAGGCCGTTCACATCGATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTccccccc                                                                               >  1:165573/1‑90 (MQ=255) aTCTTATCGAGCCCGAGGCCGTTCACATCGATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTccccccc                                                                               <  2:451870/90‑1 (MQ=255)      aTCGAGCCCGAGGCCGTTCACATCGATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGAt                                                                          >  2:264650/1‑90 (MQ=255)       tCGAGCCCGAGGCCGTTCACATCGATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATa                                                                         <  2:430671/90‑1 (MQ=255)           gCCCGAGGCCGTTCACATCGATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAAtt                                                                     >  1:235310/1‑90 (MQ=255)           gCCCGAGGCCGTTCACATCGATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAAtt                                                                     >  1:543343/1‑90 (MQ=255)           gCCCGAGGCCGTTCACATCGATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAAtt                                                                     >  1:60694/1‑90 (MQ=255)               gAGGCCGTTCACATCGATAAGGGGGGGGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTgc                                                                 >  2:536459/1‑90 (MQ=255)                  gCCGTTCACATCGATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGcc                                                              <  2:732896/90‑1 (MQ=255)                  gCCGTTCACATCGATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGcc                                                              <  1:264650/90‑1 (MQ=255)                  gCCGTTCACATCGATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGcc                                                              <  2:66109/90‑1 (MQ=255)                        cacaTCGATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTAc                                                        <  1:527468/90‑1 (MQ=255)                                    gtgggtggGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCt                                            >  1:568306/1‑90 (MQ=255)                                      gggtgggCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGcc                                                              <  1:251067/70‑1 (MQ=255)                                      gggtgggCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGcc                                                              >  2:251067/1‑70 (MQ=255)                                      gggtgggCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTaa                                          >  1:181853/1‑90 (MQ=255)                                             cGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTgccgc                                   >  2:109993/1‑90 (MQ=255)                                                ttCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTc                                <  2:60694/90‑1 (MQ=255)                                                      ttCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTgg                          <  2:744771/90‑1 (MQ=255)                                                      ttCCAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTgg                          <  1:229630/90‑1 (MQ=255)                                                         cAGCTTCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGt                       >  2:370578/1‑90 (MQ=255)                                                             ttCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTc                   <  1:687697/90‑1 (MQ=255)                                                             ttCATCAGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTc                   <  1:536459/90‑1 (MQ=255)                                                                atcaGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACt                <  1:708124/90‑1 (MQ=255)                                                                atcaGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACt                <  1:685622/90‑1 (MQ=255)                                                                 tcaGGTCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTc               <  2:181853/90‑1 (MQ=255)                                                                      tCCAGCAGATTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCa          <  1:16462/90‑1 (MQ=255)                                                                              aTTGGTCCCCCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTaaa  >  2:279508/1‑90 (MQ=255)                                                                                       |                                                                                 ATCTTATCGAGCCCGAGGCCGTTCACATCGATAAGGTGGGTGGGCGTTTCAATTTCCAGCTTCATCAGGTCCAGCAGATTGGTCCCGCCCGCGATAAATTTTGCGCCGGGTACGCGCTGAGCGCTAAGTGCCGCCTCGGCTGGGGTATTCACTCGTTCATAGGTAAA  >  NZ_CP009273/297423‑297589

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑