Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	2,818,451	T→C	E162G (GAA→GGA)	mltB ←	lytic murein transglycosylase B

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	2,818,451	0	T	C	100.0%	104.8 / NA	33	E162G (GAA→GGA)	mltB	lytic murein transglycosylase B
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (14/19);  total (14/19)

CCGCGCGGCGTGGGTAGTTAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTACTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCC  >  NZ_CP009273/2818368‑2818535                                                                                    |                                                                                     ccGCGCGGCGTGGGTAGTTAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGGTTTCCCCATCACGCGCCCCCAGCGGGGTCCAACGc                                                                                >  1:381546/1‑90 (MQ=255)   gcgcgGCGTGGGTAGTTAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTCCAACGCCg                                                                              >  2:683657/1‑90 (MQ=255)   gcgcgGCGTGGGTAGTTAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTCCAACGCCg                                                                              >  2:326233/1‑90 (MQ=255)   gcgcgGCGTGGGTAGTTAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTCCAACGCCg                                                                              >  1:735975/1‑90 (MQ=255)                  ttAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTCCAACGCCGATAATCCCGACGATa                                                               >  2:497835/1‑90 (MQ=255)                  ttAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTCCAACGCCGATAATCCCGACGATa                                                               >  2:310521/1‑90 (MQ=255)                  ttAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTCCAACGCCGATAATCCCGACGATa                                                               >  2:297131/1‑90 (MQ=255)                      aTGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTCCAACGCCGATAATCCCGACGATAAttt                                                           >  2:584621/1‑90 (MQ=255)                       tGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTCCAACGCCGATAATCCCGACGATAATTTc                                                          >  2:579775/1‑90 (MQ=255)                       tGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTCCAACGCCGATAATCCCGACGATAATTTc                                                          >  2:351878/1‑90 (MQ=255)                        gACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTCCAACGCCGATAATCCCGACGATAATTTcc                                                         <  2:766732/90‑1 (MQ=255)                           cgCGTTGCCCGCGCATCGAGGATGCGAGTTTTCCCCATCCCGCGCCCCCAGCGGGTTCCAACGCCGATAATCCCGACGATAATTTCcggc                                                      <  1:19055/89‑1 (MQ=255)                           aGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTCCAACGCCGATAATCCCGACGGTAATTTCcggc                                                      >  2:555461/1‑90 (MQ=255)                           aGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTCCAACGCCGATAATCCCGACGATAATTTCcggc                                                      >  2:121531/1‑90 (MQ=255)                               ttGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTCCAACGCCGATAATCCCGACGATAATTTCCGGCGGTa                                                  <  1:478794/90‑1 (MQ=255)                               ttGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTCCAACGCCGATAATCCCGACGATAATTTCCGGCGGTa                                                  <  2:33216/90‑1 (MQ=255)                                tGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTCCAACGCCGATAATCCCGACGATAATTTCCGGCGGTAc                                                 <  1:694151/90‑1 (MQ=255)                                  ccAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTCCAACGCCGATAATCCCGACGATAATTTCCGGCGGTACTc                                               <  2:735975/90‑1 (MQ=255)                                   cAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTCCAACGCCGATAATCCCGACGATAATTTCCGGCGGTACTcc                                              <  1:497835/90‑1 (MQ=255)                                   cAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTCCAACGCCGATAATCCCGACGATAATTTCCGGCGGTACTcc                                              <  1:297131/90‑1 (MQ=255)                                     gcgcATCGAGGATGCGAGTTTCCCCCATCCCGCGCCCCCAGCGGGTTCCAACGCCGATAATCCCGACGATAATTTCCGGCGGTACTCCAt                                            <  1:410887/90‑1 (MQ=255)                                      cgcATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTCCAACGCCGATAATCCCGACGATAATTTCcggc                                                      <  1:618468/79‑1 (MQ=255)                                      cgcATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTCCAACGCCGATAATCCCGACGATAATTTCcggc                                                      >  2:618468/1‑79 (MQ=255)                                          tCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTCCAACGCCGATAATCCCGACGATAATTTCCGGCGGTACTCCATACAcc                                       >  1:407788/1‑90 (MQ=255)                                               gATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTCCAACGCCGATAATCCCGACGATAATTTCCGGCGGTACTCCATACACCTGCCa                                  <  1:375544/90‑1 (MQ=255)                                                    gAGTTTTCCCCATCACGCGCCCCCAGCGGGTTCCAACGCCGATAATCCCGACGATAATTTCCGGCGGTACTCCATACACCTGCCAcgcgc                             <  2:243142/90‑1 (MQ=255)                                                              cgccACGCGCCCCAAGCGGGTTCCAACGCCGATAATCCGGACGATAATTTCCGCCGGTACTCCATACACCTGCCACGCGCGATTCAACGc                   <  1:180109/87‑1 (MQ=255)                                                              cATCACGCGCCCCCAGCGGGTTCCAACGCCGATAATCCCGACGATAATTTCCGGCGGTACTCCATACACCTGCCACGCGCGATTCAACGc                   <  2:268346/90‑1 (MQ=255)                                                              cATCACGCGCCCCCAGCGGGTTCCAACGCCGATAATCCCGACGATAATTTCCGGCGGTACTCCATACACCTGCCACGCGCGATTCAACGc                   <  1:681023/90‑1 (MQ=255)                                                              cATCACGCGCCCCCAGCGGGTTCCAACCCCGATAATCCCGACGATAATTTCCGGCGGTACTCCATACACCTGCCACGCGCGATTCAACGc                   <  1:557989/90‑1 (MQ=255)                                                                tCACGCGCCCCCAGCGGGTTCCAACGCCGATAATCCCGACGATAATTTCCGGCGGTACTCCATACACCTGCCACGCGCGATTCAACGCAt                 <  1:1186/90‑1 (MQ=255)                                                                            aGCGGATTCCAACCCCGATAATCCCCACGATAATTTCCGGCGGTACTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGAt     <  1:626481/90‑1 (MQ=255)                                                                               gggTTCCAACGCCGATAATCCCGACGATAATTTCCGGCGGTACTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTcc  <  1:121531/90‑1 (MQ=255)                                                                                    |                                                                                     CCGCGCGGCGTGGGTAGTTAAATGACAGCGTTGCCAGCGCATCGAGGATGCGAGTTTTCCCCATCACGCGCCCCCAGCGGGTTTCAACGCCGATAATCCCGACGATAATTTCCGGCGGTACTCCATACACCTGCCACGCGCGATTCAACGCATCTTCATACTGATTCC  >  NZ_CP009273/2818368‑2818535

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 12 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑