Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	322,687	A→G	I91T (ATC→ACC)	betA ←	choline dehydrogenase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	322,687	0	A	G	100.0%	75.6 / NA	22	I91T (ATC→ACC)	betA	choline dehydrogenase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/13);  total (9/13)

TCGAGGTAGCTCCAGTTCTCCAGACCGGGTTCTTGCGCCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCAT  >  NZ_CP009273/322600‑322768                                                                                        |                                                                                  tCGAGGTAGCTCCAGTTCTCCAGACCGGGTTCTTGCGCCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGGTc                                                                                 >  1:637181/1‑90 (MQ=255)           tCCAGTTCTCCAGACCGGGTTCTTGCGCCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGGTCAGCGACGATc                                                                       <  1:742557/90‑1 (MQ=255)                ttCTCCAGACCGGGTTCTTGCGCCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGGTCAGCGACGATCCAccc                                                                  <  2:62769/90‑1 (MQ=255)                  ctcCAGACCGGGTTCTTGCGCCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGGTCAGCGACGATCCACCCAg                                                                <  2:464867/90‑1 (MQ=255)                       gACCGGGTTCTTGCGCCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGGTCAGCGACGATCCACCCAGACCtt                                                           >  1:508071/1‑90 (MQ=255)                            ggTTCTTGCGCCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGGTCAGCGACGATCCACCCa                                                                 <  2:663606/79‑1 (MQ=255)                            ggTTCTTGCGCCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGGTCAGCGACGATCCACCCa                                                                 >  1:663606/1‑79 (MQ=255)                                     gcCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGGTCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCAc                                             <  2:131130/90‑1 (MQ=255)                                       ccAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGGTCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTc                                           <  1:373928/90‑1 (MQ=255)                                                   aTCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGGTCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGtt                               <  2:72108/90‑1 (MQ=255)                                                   aTCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGGTCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGtt                               <  1:633680/90‑1 (MQ=255)                                                    tCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGGTCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTa                              >  1:434161/1‑90 (MQ=255)                                                        gcgcATTGCCACGGATGTAGCACATGCCGTTGGTCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCa                          >  2:367952/1‑90 (MQ=255)                                                              tGCCACGGATGTAGCACATGCCGTTGGTCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACg                    <  1:276114/90‑1 (MQ=255)                                                                      aTGTAGCACATGCCGTTGGTCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGt            <  2:150391/90‑1 (MQ=255)                                                                      aTGTAGCACATGCCGTTGGTCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGt            <  2:397306/90‑1 (MQ=255)                                                                         tAGCACATGCCGTTGGTCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGt                                >  2:357359/1‑67 (MQ=255)                                                                         tAGCACATGCCGTTGGTCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGt                                <  1:357359/67‑1 (MQ=255)                                                                         tAGCACATGCCGTTGGTCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTcc         <  1:212761/90‑1 (MQ=255)                                                                              caTGCCGTTGGTCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGGTCAGGGTCCCTTTc    >  1:118646/1‑90 (MQ=255)                                                                               aTGCCGTTGGTCAGCGACGATCCACCCAGACCTTTACTGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCa   >  2:666602/1‑90 (MQ=255)                                                                                tGCCGTTGGTCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCCGGTTCCGGTTCAt  >  2:471558/1‑90 (MQ=255)                                                                                        |                                                                                  TCGAGGTAGCTCCAGTTCTCCAGACCGGGTTCTTGCGCCCAGTTATCGAGATCCAGCGCATTGCCACGGATGTAGCACATGCCGTTGATCAGCGACGATCCACCCAGACCTTTACCGCGTCCGCACTCCATGCGGCGGTTATTCATAAACGGTTCAGGTTCCGTTTCAT  >  NZ_CP009273/322600‑322768

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 19 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑