Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,785,687	G→C	G75G (GGC→GGG)	kbl ←	glycine C‑acetyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,785,687	0	G	C	100.0%	96.3 / NA	29	G75G (GGC→GGG)	kbl	glycine C‑acetyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (14/15);  total (14/15)

CCATCCCCAGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGCCCGAGATAGTTGTTGGCACAAA  >  NZ_CP009273/3785605‑3785772                                                                                   |                                                                                      ccATCCCCAGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAAc                                                                                <  2:63658/90‑1 (MQ=255) ccATCCCCAGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAAc                                                                                <  2:308152/90‑1 (MQ=255)  cATCCCCAGGAAGCCCGCCAGTTTTTGTTCAAACTCTTTATGCCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAAcc                                                                               <  1:3771/90‑1 (MQ=255)        cAGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTgaga                                                                         >  1:558133/1‑90 (MQ=255)        cAGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTgaga                                                                         >  2:306735/1‑90 (MQ=255)                  gccAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTccc                                                               >  2:314675/1‑90 (MQ=255)                  gccAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTccc                                                               >  2:21114/1‑90 (MQ=255)                            gTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTgccg                                                     >  1:507860/1‑90 (MQ=255)                               cAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAACCCGTGAGAATCCATTCCCGCCTTTGccgccg                                                  <  1:240470/90‑1 (MQ=255)                                aaGCTCTTTATGGCTGCCCTGAGTGCCGCAAATAAAACGCCCCGAAGCCACCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCcgccgc                                                 <  1:392720/90‑1 (MQ=255)                                         aTGGCTGTCCTGAGTGCCGCAAATAAACCGCCCCGAAGCCACCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAatcagatc                                        <  1:203389/90‑1 (MQ=255)                                               gTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATc                                                                      >  1:743968/1‑54 (MQ=255)                                               gTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATc                                                                      <  2:743968/54‑1 (MQ=255)                                               gTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGgatg                                  >  1:771764/1‑90 (MQ=255)                                               gTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGgatg                                  >  1:511178/1‑90 (MQ=255)                                               gTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGgatg                                  >  1:49864/1‑90 (MQ=255)                                               gTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGgatg                                  >  1:494054/1‑90 (MQ=255)                                                    gAGTGCCGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCg                             >  2:439014/1‑90 (MQ=255)                                                         ccGCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGc                        >  1:526072/1‑90 (MQ=255)                                                           gCAAATAAAACGCCCCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGccc                      <  1:436297/90‑1 (MQ=255)                                                           gCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGccc                      >  1:114867/1‑90 (MQ=255)                                                           gCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGccc                      <  2:435545/90‑1 (MQ=255)                                                           gCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGccc                      <  2:518329/90‑1 (MQ=255)                                                           gCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGccc                      <  2:578896/90‑1 (MQ=255)                                                           gCAAATAAAACGCACCGAAGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGccc                      >  1:187806/1‑90 (MQ=255)                                                           gCAAATAAAACGCACCGAAGCCACCCCGAAACCGTGAGAATCCATCCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGccc                      <  1:334222/90‑1 (MQ=255)                                                                             aGCCATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGCCCGAGATAGTTGTTGGcaca    <  2:85583/90‑1 (MQ=255)                                                                              gCCATCCCGAAACCGTGAGAACCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGCCCGAGATAGTTGTTGGCACaa   <  1:685614/90‑1 (MQ=255)                                                                               ccATCCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGCCCGAGATAGTTGTTGGCACaaa  <  1:657519/90‑1 (MQ=255)                                                                                   |                                                                                      CCATCCCCAGGAAGGCCGCCAGTTTTTGTTCAAGCTCTTTATGGCTGTCCTGAGTGCCGCAAATAAAACGCACCGAAGCCATGCCGAAACCGTGAGAATCCATTCCCGCCTTTGCCGCCGCAATCAGATCAGGATGATTCGCCAGCCCGAGATAGTTGTTGGCACAAA  >  NZ_CP009273/3785605‑3785772

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑