Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	3,786,467	T→C	I193V (ATT→GTT)	yibB ←	protein YibB

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	3,786,467	0	T	C	100.0%	193.6 / NA	54	I193V (ATT→GTT)	yibB	protein YibB
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (39/15);  total (39/15)

CCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAATCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAATGG  >  NZ_CP009273/3786386‑3786551                                                                                  |                                                                                     ccAGAACTAATTTATAGAATTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAAt                                                                              <  1:326112/90‑1 (MQ=255)    gAACTAATTTATAGAATTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCaa                                                                           <  1:12213/90‑1 (MQ=255)         aaTTTATAGAATTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAAcc                                                                      >  2:755563/1‑90 (MQ=255)         aaTTTATAGAATTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAAcc                                                                      >  2:278914/1‑90 (MQ=255)          aTTTATAGAATTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCt                                                                     <  1:482456/90‑1 (MQ=255)            ttATAGAATTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCtgt                                                                   <  2:478990/90‑1 (MQ=255)                   aTTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTgag                                                            >  2:333738/1‑90 (MQ=255)                   aTTCTTTCCATTTATGCTGTGAACCAACAAGTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGttgttgcg                                                            >  2:675495/1‑88 (MQ=255)                        ttCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGAtgtg                                                       <  1:415693/90‑1 (MQ=255)                        ttCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGAtgtg                                                       >  2:241146/1‑90 (MQ=255)                         tCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGa                                                      <  1:719607/90‑1 (MQ=255)                             tttATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGt                                                  >  2:546228/1‑90 (MQ=255)                             tttATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGt                                                  <  1:546228/90‑1 (MQ=255)                              ttATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTa                                                 <  1:645090/90‑1 (MQ=255)                                  gCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGcc                                             <  2:570227/90‑1 (MQ=255)                                     gtgAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCtt                                          >  2:688721/1‑90 (MQ=255)                                     gtgAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCtt                                          >  1:567374/1‑90 (MQ=255)                                     gtgAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCtt                                          >  1:543282/1‑90 (MQ=255)                                       gAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCtttt                                        >  1:6416/1‑90 (MQ=255)                                       gAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCtttt                                        >  1:753137/1‑90 (MQ=255)                                            aacaaTTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTtat                                   <  2:621203/90‑1 (MQ=255)                                             acaaTTGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTtata                                  <  2:753137/90‑1 (MQ=255)                                                 ttGCGCCCCCAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGa                              >  1:556037/1‑90 (MQ=255)                                                        cccAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATg                       <  2:127347/90‑1 (MQ=255)                                                        cccAATAATATATACATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATg                       >  2:288440/1‑90 (MQ=255)                                                            ataataTTTACATGATTGCCAACCATTAAATTAAAAACCTGTTGTTGAGATGTTACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATc                   <  1:8876/90‑1 (MQ=255)                                                                 atataCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACt              >  1:633353/1‑90 (MQ=255)                                                                  tataCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACtt             >  2:581318/1‑90 (MQ=255)                                                                  tataCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACtt             >  2:685758/1‑90 (MQ=255)                                                                  tataCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACtt             >  1:245167/1‑90 (MQ=255)                                                                  tataCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACtt             >  1:748553/1‑90 (MQ=255)                                                                  tataCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACtt             >  2:626479/1‑90 (MQ=255)                                                                    taCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTa                                                 <  2:474764/52‑1 (MQ=255)                                                                    taCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTa                                                 >  1:474764/1‑52 (MQ=255)                                                                    taCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTtc           >  1:74905/1‑90 (MQ=255)                                                                    taCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTtc           >  2:68167/1‑90 (MQ=255)                                                                    taCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTtc           >  2:486618/1‑90 (MQ=255)                                                                    taCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTtc           >  2:488780/1‑90 (MQ=255)                                                                    taCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTtc           >  2:513236/1‑90 (MQ=255)                                                                    taCATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTtc           >  2:565444/1‑90 (MQ=255)                                                                      cATGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTtcat         >  2:380761/1‑90 (MQ=255)                                                                        tGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTTTAGTGAAAAGATGCATCTTACTTtcatca       >  1:683618/1‑90 (MQ=255)                                                                        tGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTtcatca       >  2:341982/1‑90 (MQ=255)                                                                        tGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTtcatca       >  1:127474/1‑90 (MQ=255)                                                                        tGATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTtcatca       >  1:273607/1‑90 (MQ=255)                                                                         gATTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCaa      >  1:85744/1‑90 (MQ=255)                                                                          aTTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGGAAAGATGCATCTTACTTTCATCaaa     >  1:721751/1‑90 (MQ=255)                                                                          aTTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCaaa     >  1:118113/1‑90 (MQ=255)                                                                          aTTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCaaa     >  1:42689/1‑90 (MQ=255)                                                                          aTTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCaaa     <  1:341982/90‑1 (MQ=255)                                                                          aTTGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCaaa     >  1:104785/1‑90 (MQ=255)                                                                           ttGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAAt    >  2:346978/1‑90 (MQ=255)                                                                           ttGCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAAt    >  2:9545/1‑90 (MQ=255)                                                                             gCCAACCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAATgg  >  2:350259/1‑90 (MQ=255)                                                                                  |                                                                                     CCAGAACTAATTTATAGAATTCTTTCCATTTATGCTGTGAACCAACAATTGCGCCCCCAATAATATATACATGATTGCCAATCATGAAATCAAAAACCTGTTGTTGAGATGTGACAGTAAGCCCTTTTTTTATAGTGAAAAGATGCATCTTACTTTCATCAAATGG  >  NZ_CP009273/3786386‑3786551

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑