Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	49,903	T→A	D27E (GAT→GAA)	folA →	type 3 dihydrofolate reductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	49,903	0	T	A	100.0%	77.9 / NA	25	D27E (GAT→GAA)	folA	type 3 dihydrofolate reductase
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base A (14/11);  total (14/11)

CAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTC  >  NZ_CP009273/49828‑49976                                                                            |                                                                          cAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTaa                                                             >  1:463544/1‑90 (MQ=255)  aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTaaa                                                            >  2:348799/1‑90 (MQ=255)  aGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTaaa                                                            >  2:703933/1‑90 (MQ=255)      tGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCA‑GCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCaa                                                       <  2:350595/90‑1 (MQ=255)       gATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCaa                                                       <  2:331358/90‑1 (MQ=255)        aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAac                                                      <  2:436575/90‑1 (MQ=255)        aTTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAac                                                      <  1:712832/90‑1 (MQ=255)            cggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCt                                                  <  2:105315/90‑1 (MQ=255)             ggcgTTAGCGGTAGATCGCGTTATCGGCATGGGAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCtt                                                 >  1:579332/1‑90 (MQ=255)             ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGGACCTGCCTGCCGAACTCGCCTGGTTTAAAAGCAACACCtt                                                 >  1:257251/1‑90 (MQ=255)             ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCtt                                                 >  2:658309/1‑90 (MQ=255)             ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAAATCGCCTGGTTTAAACGCAACACCtt                                                 >  1:589686/1‑90 (MQ=255)             ggcgTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGGGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCtt                                                 >  2:265010/1‑90 (MQ=255)                 ttAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGGTTAAACGCAACACCTtaaat                                             >  1:643374/1‑90 (MQ=255)                   aGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTtaaataa                                           <  1:642179/90‑1 (MQ=255)                              gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAAccc                                       <  2:129303/83‑1 (MQ=255)                              gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAAccc                                       >  1:129303/1‑83 (MQ=255)                              gcgTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAAAGCAACACCTTAAATAAACCCGTGATTa                                >  1:376533/1‑90 (MQ=255)                                  tATCGGCATGGAAACCGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATggg                            <  1:525123/90‑1 (MQ=255)                                  tATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCtt                                                 <  1:375925/69‑1 (MQ=255)                                  tATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCtt                                                 >  2:375925/1‑69 (MQ=255)                                         aTGGAAAACGCCATCCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCAt                     <  1:183322/90‑1 (MQ=255)                                                     aTGCCGTGGAACCTGCCTGCCGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGaatca         >  2:759916/1‑90 (MQ=255)                                                                   gcctgccGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtc  >  2:160932/1‑83 (MQ=255)                                                                   gcctgccGAACTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGgtc  <  1:160932/83‑1 (MQ=255)                                                                            |                                                                          CAGTCTGATTGCGGCGTTAGCGGTAGATCGCGTTATCGGCATGGAAAACGCCATGCCGTGGAACCTGCCTGCCGATCTCGCCTGGTTTAAACGCAACACCTTAAATAAACCCGTGATTATGGGCCGCCATACCTGGGAATCAATCGGTC  >  NZ_CP009273/49828‑49976

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑