Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	4,613,222	C→G	R238P (CGC→CCC)	lplA ←	lipoate‑‑protein ligase LplA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	4,613,222	0	C	G	100.0%	58.9 / NA	18	R238P (CGC→CCC)	lplA	lipoate‑‑protein ligase LplA
Reads supporting (aligned to +/- strand):  ref base C (0/0);  new base G (10/8);  total (10/8)

ACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGCGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCA  >  NZ_CP009273/4613141‑4613299                                                                                  |                                                                              aCGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGGGGGCAAAg                                                                       >  1:574825/1‑90 (MQ=255)      gccCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGGGGGCAAAGGTTTc                                                                  >  2:640349/1‑90 (MQ=255)                aaGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGGGGGCAAAGGTTTCGGCGAAGttt                                                        >  1:701760/1‑90 (MQ=255)                    gTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGGGGGCAAAGGTTTCGGCGAAGTTTGGCa                                                    <  1:761460/90‑1 (MQ=255)                        aTCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGGGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTc                                                <  1:135454/90‑1 (MQ=255)                                   gCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGGGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGt                                     <  2:342793/90‑1 (MQ=255)                                      agaATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGGGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCg                                  <  1:435020/90‑1 (MQ=255)                                          tGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGGGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGga                              >  1:457467/1‑90 (MQ=255)                                          tGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGGGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGga                              >  1:599627/1‑90 (MQ=255)                                              ggAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGGGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAgatg                          >  1:475399/1‑90 (MQ=255)                                                            gTTCCATTCCCAGCTCCTCTGGGGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCAc            <  1:91152/90‑1 (MQ=255)                                                                cATTCCCAGCTACTCTGGGGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACgcgc        <  1:427451/90‑1 (MQ=255)                                                                 aTTCCCAGCTACTCTGGGGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCt       <  1:177747/90‑1 (MQ=255)                                                                  ttCCCAGCTACTCTGGGGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTc      <  2:649850/90‑1 (MQ=255)                                                                    cccAGCTACTCTGGGGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGc    >  2:434191/1‑90 (MQ=255)                                                                      cAGCTACTCTGGGGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCa  >  1:447673/1‑90 (MQ=255)                                                                      cAGCTACTCTGGGGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCa  >  1:22609/1‑90 (MQ=255)                                                                      cAGCTACTCTGGGGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCa  >  1:146816/1‑90 (MQ=255)                                                                                  |                                                                              ACGCCGCCCCAGGTAAAGCGTTCATCCAGCAGATGCGAGAATGCCGGAGCCTGACCGAAGTTCCATTCCCAGCTACTCTGGCGGGCAAAGGTTTCGGCGAAGTTTGGCAAGTCTGGCGTTTTGTTCGGGGAGATGATTTCCGCTTCCACGCGCTCGCCA  >  NZ_CP009273/4613141‑4613299

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 26 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑