Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	465,464	T→C	S380P (TCG→CCG)	mdlA →	SmdA family multidrug ABC transporter permease/ATP‑binding protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	465,464	0	T	C	100.0%	86.2 / NA	26	S380P (TCG→CCG)	mdlA	SmdA family multidrug ABC transporter permease/ATP‑binding protein
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (10/16);  total (10/16)

GGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACT  >  NZ_CP009273/465382‑465540                                                                                   |                                                                             ggAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTCCCCTGTTGCCGCTctg                                                                       <  2:148869/90‑3 (MQ=255)   aaaaCGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGCCGCTCATTc                                                                     <  2:516610/90‑1 (MQ=255)                              gTCAGATGCTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGCCGCTCATTCAGCGTCATTTCGACGTCAGCGAggggg                                          >  1:751615/1‑90 (MQ=255)                                  gATGCTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAGGTACCCTGTTGCCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGatat                                      <  1:142315/90‑1 (MQ=255)                                  gATGCTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGCCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGatat                                      >  2:708736/1‑90 (MQ=255)                                  gATGCTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGCCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGatat                                      <  2:159523/90‑1 (MQ=255)                                        gggTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGCCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCtt                                >  1:281720/1‑90 (MQ=255)                                        gggTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGCCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCtt                                >  2:170158/1‑90 (MQ=255)                                         ggTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCTTGTTGCCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCttt                               <  2:721719/90‑1 (MQ=255)                                          gTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGCCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTc                              <  1:337103/90‑1 (MQ=255)                                          gTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGCCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTc                              <  1:128960/90‑1 (MQ=255)                                          gTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGCCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTc                              <  1:280725/90‑1 (MQ=255)                                          gTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGCCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTc                              <  2:346668/90‑1 (MQ=255)                                            aTCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGCCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCAt                            >  1:647903/1‑90 (MQ=255)                                            aTCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGCCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCAt                            <  2:712817/90‑1 (MQ=255)                                            aTCTGCGCGCGGAAAGGATACCGCCAAAGAACCCTGTTGCCGAACATTCCGCGCCATTTCGACGTCAGCGCGGGGGATATCCGCTTTCAt                            >  1:653459/1‑90 (MQ=255)                                                  gggCCGACTGGTTCCGGCAAAAGTACCCTGTTGCCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATAtt                      >  1:470966/1‑90 (MQ=255)                                                  gggCCGACTGGTTCCGGCAAAAGTACCCTGTTGCCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATAtt                      <  2:537798/90‑1 (MQ=255)                                                       gACTGGTTCCGGCAAAAGTACCCTGTTGCCGCTCATTCAGCGTCATTTCGACGTc                                                    >  2:94326/1‑55 (MQ=255)                                                       gACTGGTTCCGGCAAAAGTACCCTGTTGCCGCTCATTCAGCGTCATTTCGACGTc                                                    <  1:94326/55‑1 (MQ=255)                                                           ggTTCCGGCAAAAGTACCCTGTTGCCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACg             <  2:593345/90‑1 (MQ=255)                                                             ttCCGGCAAAAGTACCCTGTTGCCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGaa           >  1:715943/1‑90 (MQ=255)                                                             ttCCGGCAAAAGTACCCTGTTGCCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGaa           <  1:67128/90‑1 (MQ=255)                                                             ttCCGGCAAAAGTACCCTGTTGCCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGaa           <  1:662035/90‑1 (MQ=255)                                                                   cAAAAGTACCCTGTTGCCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACa     <  2:67355/90‑1 (MQ=255)                                                                      aaGTACCCTGTTGCCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACt  >  1:70271/1‑90 (MQ=255)                                                                                   |                                                                             GGAAAACGTCAATTTCGCCCTGAAACCCGGTCAGATGCTGGGTATCTGCGGGCCGACTGGTTCCGGCAAAAGTACCCTGTTGTCGCTCATTCAGCGTCATTTCGACGTCAGCGAGGGGGATATTCGCTTTCATGATATTCCTCTGACGAAGTTACAACT  >  NZ_CP009273/465382‑465540

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑