Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	568,752	A→G	G71G (GGA→GGG)	ybcO →	DUF1364 domain‑containing protein

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	568,752	0	A	G	100.0%	92.7 / NA	27	G71G (GGA→GGG)	ybcO	DUF1364 domain‑containing protein
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (6/21);  total (6/21)

AACCGCCAGACCTGATTGCCACCATTGCATGTTCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGATATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTATCTGGCTGAAAGAGGGGGTTATTAAGGCGTGAATACCTAC  >  NZ_CP009273/568670‑568838                                                                                   |                                                                                       aaCCGCCAGACCTGATTGCCACCATTGCATGTTCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCaa                                                                                 >  1:325740/1‑90 (MQ=255)           ccTGATTGCCACCATTGCATGTTCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATgcgc                                                                       <  2:565688/90‑1 (MQ=255)           ccTGATTGCCACCATTGCATGTTCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATgcgc                                                                       <  2:341089/90‑1 (MQ=255)           ccTGATTGCCACCATTGCATGTTCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATgcgc                                                                       <  2:183194/90‑1 (MQ=255)           ccTGATTGCCACCATTGCATGTTCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATgcgc                                                                       <  1:483580/90‑1 (MQ=255)             tGATTGCCACCATTGCATGTTCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATgcgcgc                                                                     <  2:174864/90‑1 (MQ=255)              gATTGCCACCATTGCATGTTCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCt                                                                    >  2:766803/1‑90 (MQ=255)                  gCCACCATTGCATGTTCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGaa                                                                <  2:181071/90‑1 (MQ=255)                      ccaTTGCATGTTCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTa                                                            <  1:733923/90‑1 (MQ=255)                        aTTGCATGTTCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATg                                                          <  2:454407/90‑1 (MQ=255)                        aTTGCATGTTCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATg                                                          <  2:325740/90‑1 (MQ=255)                           gCATGTTCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCg                                                       <  1:523364/90‑1 (MQ=255)                           gCATGTTCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCg                                                       <  2:447042/90‑1 (MQ=255)                                ttCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCg                                                       >  2:330326/1‑85 (MQ=255)                                ttCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCg                                                       <  1:330326/85‑1 (MQ=255)                                    gcctgccACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAg                                              >  2:765850/1‑90 (MQ=255)                                    gcctgccACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAg                                              >  1:189336/1‑90 (MQ=255)                                    gcctgccACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGGGAGGCAAAAGAAGGCGCGCTGGAGGGTATGGCGAGAACACAg                                              >  1:399735/1‑90 (MQ=255)                                                 aaaTCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTATCTGGCTGa                                 <  1:40483/90‑1 (MQ=255)                                                 aaaTCGACCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTATCTGGCTGa                                 <  2:689334/90‑1 (MQ=255)                                                      gcccgccgCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTATCTGGCTGAAagag                            <  1:649265/88‑1 (MQ=255)                                                       aCCGCCGCACGCATTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTATCTGGCTGAAAGAgg                           <  1:401857/90‑1 (MQ=255)                                                                cgcaTTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTATCTGGCTGAAAGAGGGGGttatta                  <  2:28391/90‑1 (MQ=255)                                                                   aTTTTGTTGACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTATCTGGCTGAAAGAGGGGGTTATTAAgg               <  2:66429/90‑1 (MQ=255)                                                                         ttgACGCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTATCTGGGTGAAAGAGGGGGTTATTAAGGCGTGaa         <  1:588134/90‑1 (MQ=255)                                                                              gCTGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTATCTGGCTGAAAGAGGGGGTTATTAAGGCGTGAATACCt    <  1:233591/90‑1 (MQ=255)                                                                                tGGGTATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTATCTGGCTGAAAGAGGGGGTTATTAAGGCGTGAATACCTAc  <  2:109235/90‑1 (MQ=255)                                                                                   |                                                                                       AACCGCCAGACCTGATTGCCACCATTGCATGTTCTGCCTGCCACGACGAAATCGACCGCCGCACGCATTTTGTTGACGCTGGATATGCAAAAGAATGCGCGCTGGAAGGTATGGCGAGAACACAGGTTATCTGGCTGAAAGAGGGGGTTATTAAGGCGTGAATACCTAC  >  NZ_CP009273/568670‑568838

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 18 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑