Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	661,892	A→C	G594G (GGT→GGG)	mrdA ←	peptidoglycan DD‑transpeptidase MrdA

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	661,892	0	A	C	100.0%	129.8 / NA	37	G594G (GGT→GGG)	mrdA	peptidoglycan DD‑transpeptidase MrdA
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base C (19/18);  total (19/18)

TTCCGCAGGCAGATCGGTGTTGTTATCACCCAGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCACCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGTTTGTGGTCACGTAAACGCTC  >  NZ_CP009273/661805‑661978                                                                                        |                                                                                       ttCCGCAGGCAGATCGGTGTTGTTATCACCCAGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCaccccc                                                                                      <  2:458653/90‑1 (MQ=255)   ccGCAGGCAGATCGGTGTTGTTATCCCCCAGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGt                                                                                    <  1:737030/90‑1 (MQ=255)    cGCAGGCAGATCGGTGTTGTTATCACCCAGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGtt                                                                                   <  2:383192/90‑1 (MQ=255)                             cccAGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACt                                                          >  1:475959/1‑90 (MQ=255)                               cAGCATAATGTGGTCGAGGTTCTGGCGCATCAGTGTCCCACCCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTg                                                        <  1:601429/90‑1 (MQ=255)                                aGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGc                                                       >  2:734949/1‑90 (MQ=255)                                aGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGc                                                       >  2:280940/1‑90 (MQ=255)                                aGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGc                                                       >  2:168195/1‑90 (MQ=255)                                aGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGc                                                       >  2:15337/1‑90 (MQ=255)                                aGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGc                                                       >  1:604021/1‑90 (MQ=255)                                 gCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCg                                                      >  1:659011/1‑90 (MQ=255)                                  cATAATGGGGTCGAGGATTTGGCGCATCCGTGAACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCgg                                                     <  1:337330/90‑1 (MQ=255)                                    tAATGTGGTCGAGGATCTGGCGCATCAGTGTCCCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGAt                                                   <  1:580332/90‑1 (MQ=255)                                    tAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGAt                                                   <  1:424812/90‑1 (MQ=255)                                    tAATGTGGTCGAGGATCGGGCGCATCAGTGTCCCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGAt                                                   <  1:603484/90‑1 (MQ=255)                                      aTGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGAttg                                                 >  2:464297/1‑90 (MQ=255)                                           gTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTa                                            >  2:158050/1‑90 (MQ=255)                                           gTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTa                                            >  1:139578/1‑90 (MQ=255)                                           gTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTa                                            >  2:671841/1‑90 (MQ=255)                                           gTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTa                                            >  2:478212/1‑90 (MQ=255)                                           gTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTa                                            >  1:377329/1‑90 (MQ=255)                                            tCGAGGATCTGGCGCATCAGTGTACCACCCGCCGGACCCGCCCCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTAt                                           <  2:102460/90‑1 (MQ=255)                                            tCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTAt                                           <  1:464297/90‑1 (MQ=255)                                            tCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTAt                                           <  2:642727/90‑1 (MQ=255)                                                ggATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGgcg                                       >  1:367591/1‑90 (MQ=255)                                                     tGGCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAg                                  >  1:688851/1‑90 (MQ=255)                                                      ggCGCATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAgg                                 <  2:688851/90‑1 (MQ=255)                                                       gcgcATCAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGc                                <  2:276998/90‑1 (MQ=255)                                                        cgcATAAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCg                               <  1:671841/90‑1 (MQ=255)                                                             cAGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGtcat                          <  2:367591/90‑1 (MQ=255)                                                              aGTGTACCAACCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGtcatc                         <  2:241923/90‑1 (MQ=255)                                                                      aaCCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCgg                                                     >  2:333071/1‑54 (MQ=255)                                                                      aaCCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCgg                                                     <  1:333071/54‑1 (MQ=255)                                                                      aaCCGCCGGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGTTtgtg                 >  1:401067/1‑90 (MQ=255)                                                                         cgccgGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGTTTGTGGTc              <  2:139578/90‑1 (MQ=255)                                                                         cgccgGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGTTTGTGGTc              >  1:398926/1‑90 (MQ=255)                                                                         cgccgGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGTTTGTGGTc              <  1:390030/90‑1 (MQ=255)                                                                         cgccgGACCCGCACCCCCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGTTTGTGGTc              >  2:132138/1‑90 (MQ=255)                                                                                     acccccGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGTTTGTGGTCACGTAAACGCTc  <  1:430389/90‑1 (MQ=255)                                                                                        |                                                                                       TTCCGCAGGCAGATCGGTGTTGTTATCACCCAGCATAATGTGGTCGAGGATCTGGCGCATCAGTGTACCAACCGCCGGACCCGCACCACCGTTCTCCAGAATCATGGCGACAGCCACTTGCGGATTGTTGTATGGCGCAAAGGCGGTCATCAGTTTGTGGTCACGTAAACGCTC  >  NZ_CP009273/661805‑661978

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑