| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 716,324 | C→T | intergenic (‑46/+188) | BW25113_RS26090 ← / ← kdpE | leader peptide SpeFL/two‑component system response regulator KdpE |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 716,324 | 0 | C | T | 88.9% | 103.9 / 4.2 | 36 | intergenic (‑46/+188) | BW25113_RS26090/kdpE | leader peptide SpeFL/two‑component system response regulator KdpE |
| Reads supporting (aligned to +/- strand): ref base C (1/3); new base T (9/23); total (10/26) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 5.70e-01 | |||||||||||
TCCGCCTTATATGGGGCATAGTGCGGCTGTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAA > NZ_CP009273/716239‑716407 | tCCGCCTTATATGGGGCATAGTGCGGCTGTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCTAGGa > 1:221063/1‑90 (MQ=255) gCCTTATATGGGGCATAGTGCGGCTGTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCTAGGAAAt < 2:438051/90‑1 (MQ=255) gCCTTATATGGGGCATAGTGCGGCTGTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCTAGGAAAt < 1:480302/90‑1 (MQ=255) cTTATATGGGGCATAGTGCGGCTGTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCTAGGAAATAc < 1:360788/90‑1 (MQ=255) cTTATATGGGGCATAGTGCGGCTGTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCTAGGAAATAc < 1:374471/90‑1 (MQ=255) atatGGGGCATAGTGCGGCTGTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCTAGGAAATACGGt < 1:139019/90‑1 (MQ=255) atatGGGGCATAGTGCGGCTGTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCTAGGAAATACGGt < 1:205223/90‑1 (MQ=255) tGGGGCATAGTGCGGCTGTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCTAGGAAATACGGTTGa < 2:565994/90‑1 (MQ=255) tGGGGCATAGTGCGGCTGTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCTAGGAAATACGGTTGa < 1:406293/90‑1 (MQ=255) gggCATAGTGCGGCTGTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCTAGGAAATACGGTTGa < 1:193591/88‑1 (MQ=255) gggCATAGTGCGGCTGTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCTAGGAAATACGGTTGa > 2:193591/1‑88 (MQ=255) gggCATAGTGCGGCTGTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCTAGGAAATACGGTTGAAc > 2:85467/1‑90 (MQ=255) gggCATAGTGCGGCTGTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCTAGGAAATACGGTTGAAc > 1:267683/1‑90 (MQ=255) ggCATAGTGCGGCTGTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCTAGGAAAt > 2:594939/1‑79 (MQ=255) ggCATAGTGCGGCTGTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCTAGGAAAt < 1:594939/79‑1 (MQ=255) ggCATAGTGCGGCTGTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCTAGGAAATACGGTTGAACt > 1:548766/1‑90 (MQ=255) cATAGTGCGGCTGTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCTAGGAAATACGGTTGAACtgt > 1:48006/1‑90 (MQ=255) tAGTGCGGCTGTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCTAGGAAATACGGTTGAACTGTGa < 2:267683/90‑1 (MQ=255) tAGTGCGGCTGTTATTTTCCATTTAATTTACCTTTCCCGATGTGAATGAAGGTGAAGATATTCTATCTAGGAAATACGGTTGAACTGTGa > 1:7126/1‑90 (MQ=255) aGTGCGGCTGTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCTAGGAAATACGGTTGAACTGTGaa < 2:293068/90‑1 (MQ=255) gCTGTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCTAGGAAATACGGTTGAACTGTGAAGGTGAt < 1:676360/90‑1 (MQ=255) gCTGTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGAt > 1:137146/1‑90 (MQ=255) cTGTTATTTTCCATTTAATTTACCTTTCCCGATGTGAATGAAGGTGAAGATATTCTATCTAGGAAATACGGTTGAACTGTGAAGGTGATc < 2:7126/90‑1 (MQ=255) gTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCTAGGAAATACGGTTGAACTGTGAAGGTGATCaa > 2:608713/1‑90 (MQ=255) tttaatttaCCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCTAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGAt < 2:702193/90‑1 (MQ=255) ttaatttaCCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCTAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATc < 2:221063/90‑1 (MQ=255) ttaatttaCCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCTAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATc < 2:548766/90‑1 (MQ=255) tttCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAatt < 1:352670/90‑1 (MQ=255) tttCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAatt < 2:137146/90‑1 (MQ=255) tttCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAatt < 1:465335/90‑1 (MQ=255) tGATGTGAATGAAGGTGAAGATATTCTATCTAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAatttattt < 2:48006/90‑1 (MQ=255) aatgaaGGTGAAGATATTCTATCTAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAatgatg < 1:298827/90‑1 (MQ=255) aatgaaGGTGAAGATATTCTATCTAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAatgatg < 2:562585/90‑1 (MQ=255) aatgaaGGTGAAGATATTCTATCTAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAatgatg < 2:590594/90‑1 (MQ=255) aatgaaGGTGAAGATATTCTATCTAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAatgatg < 2:678489/90‑1 (MQ=255) gATATTCTATCTAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTc < 1:383463/90‑1 (MQ=255) tctatctaGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTaa < 1:608713/90‑1 (MQ=255) tctatctaGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTaa < 2:731970/90‑1 (MQ=255) | TCCGCCTTATATGGGGCATAGTGCGGCTGTTATTTTCCATTTAATTTACCTTTCCTGATGTGAATGAAGGTGAAGATATTCTATCCAGGAAATACGGTTGAACTGTGAAGGTGATCAATAAAAAATGATCAATCTTAATTTATTTAATGATGAGCTTTTTACTCAGTAA > NZ_CP009273/716239‑716407 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 28 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |