Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	886,189	A→G	G7G (GGT→GGC)	grxA ←	glutaredoxin 1

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	886,189	0	A	G	100.0%	64.8 / NA	19	G7G (GGT→GGC)	grxA	glutaredoxin 1
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (9/10);  total (9/10)

GATACTGAAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATGATCA  >  NZ_CP009273/886110‑886246                                                                                |                                                          gatactgaAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCAAAAATaa                                                 >  1:161107/1‑90 (MQ=255) gatactgaAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCAAAAATaa                                                 >  2:428692/1‑90 (MQ=255) gatactgaAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCAAAAATaa                                                 >  2:366651/1‑90 (MQ=255) gatactgaAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCAAAAATaa                                                 <  2:161107/90‑1 (MQ=255) gatactgaAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCAAAAATaa                                                 <  1:366651/90‑1 (MQ=255)      tgaAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCAAAAATAACGGtt                                            <  2:139668/90‑1 (MQ=255)                           cTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCATa                       <  2:459152/90‑1 (MQ=255)                                    tcAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCa                                                        <  1:689539/48‑1 (MQ=255)                                    tcAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCa                                                        >  2:689539/1‑48 (MQ=255)                                    tcAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGc              >  2:250131/1‑90 (MQ=255)                                    tcAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGc              >  2:478550/1‑90 (MQ=255)                                          aGATCTTTTGCACACACACAGTAAGGGCAACCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCATa                       >  2:152085/1‑75 (MQ=255)                                          aGATCTTTTGCACACACACAGTAAGGGCAACCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCATa                       <  1:152085/75‑1 (MQ=255)                                               ttttGCACGCACACAGTAAGGGCAACCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATGATc   <  1:400543/90‑1 (MQ=255)                                                tttGCACGCACACAGTAAGGGCAACCCGAACGGCCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATGATCa  <  2:655750/90‑1 (MQ=255)                                                     acgcacACAGTAAGGGCAACCCGAACGGCCAAAAATAACGGTTTGc                                         <  1:58003/46‑1 (MQ=255)                                                     acgcacACAGTAAGGGCAACCCGAACGGCCAAAAATAACGGTTTGc                                         >  2:58003/1‑46 (MQ=255)                                                                      aaCCCGAACGGCCAAAAATAACGGTTTGCATTATTtctc                               >  1:709268/1‑39 (MQ=39)                                                                      aaCCCGAACGGCCAAAAATAACGGTTTGCATTATTtctc                               <  2:709268/39‑1 (MQ=39)                                                                                |                                                          GATACTGAAAATCATCGCGTTCATTGCTCAATTTCTCAGCCAGATCTTTTGCACGCACACAGTAAGGGCAACCCGAACGACCAAAAATAACGGTTTGCATTATTTCTCTCCTCATAGATTTATGCCTGTAATGATCA  >  NZ_CP009273/886110‑886246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 36 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑