Predicted mutation
evidence seq id position mutation annotation gene description
RA NZ_CP009273 1,153,703 A→T I127F (ATC→TTC)  ptsG → PTS glucose transporter subunit IIBC

Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,153,7030AT100.0% 58.5 / NA 18I127F (ATC→TTC) ptsGPTS glucose transporter subunit IIBC
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (10/8);  total (10/8)

ACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATC  >  NZ_CP009273/1153618‑1153789
                                                                                     |                                                                                      
aCTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGc                                                                                    >  2:98479/1‑90 (MQ=255)
        tGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTACa                                                                            <  2:535881/90‑1 (MQ=255)
             ttACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTACATGttt                                                                       <  2:510015/90‑1 (MQ=255)
              tACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTa                                                                              <  1:358314/82‑1 (MQ=255)
              tACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTa                                                                              >  2:358314/1‑82 (MQ=255)
                         gaaATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTACATGTTTAACCGTTTCTAc                                                           <  1:761406/90‑1 (MQ=255)
                             tCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTACATGTTTAACCGTTTCTACCGTa                                                       >  2:185051/1‑90 (MQ=255)
                                     aaaCACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTg                                               <  2:289504/90‑1 (MQ=255)
                                          ccTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGc                                                                                    <  1:533358/48‑1 (MQ=255)
                                          ccTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGTTCGc                                                                                    >  2:533358/1‑48 (MQ=255)
                                                       ggCGTACTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGcttc                             >  1:729889/1‑90 (MQ=255)
                                                           tACTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTg                         >  1:91770/1‑90 (MQ=255)
                                                            aCTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGc                        <  2:400982/90‑1 (MQ=255)
                                                            aCTCGGAGGGATTATCTCCGGTGCGTTCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGc                        >  2:641735/1‑90 (MQ=255)
                                                                      aTTATCTCCGGTGCGTTCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGc              >  1:366854/1‑90 (MQ=255)
                                                                               ggTGCGTTCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCg     >  1:516730/1‑90 (MQ=255)
                                                                               ggTGCGTTCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCg     >  1:224743/1‑90 (MQ=255)
                                                                                  gCGTTCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATc  <  2:138075/90‑1 (MQ=255)
                                                                                     |                                                                                      
ACTGGTACTGCATTTACCTGCTGAAGAAATCGCCTCTAAACACCTGGCGGATACTGGCGTACTCGGAGGGATTATCTCCGGTGCGATCGCAGCGTACATGTTTAACCGTTTCTACCGTATTAAGCTGCCTGAGTATCTTGGCTTCTTTGCCGGTAAACGCTTTGTGCCGATC  >  NZ_CP009273/1153618‑1153789

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap:     Deleted base: