Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,167,328	T→C	E698G (GAA→GGA)	mfd ←	transcription‑repair coupling factor

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,167,328	0	T	C	100.0%	33.4 / NA	11	E698G (GAA→GGA)	mfd	transcription‑repair coupling factor
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (6/5);  total (6/5)

ATCAGCAGGCCTAAATCTTTAAACTTGACGTCACTTTGCAGCAGTTTGTGCGTACCGATCAGAATATCGATTTTCCCTTCCGCCACTTCCGCAAGGATTTGCGTCTGCTCTTTGGCGCTGCGGAAACGGGAGATCATTTCGATACGTACCGGCCAGTTGGCGAA  >  NZ_CP009273/1167241‑1167404                                                                                        |                                                                             aTCAGCAGGCCTAAATCTTTAAACTTGACGTCACTTTGCAGCAGTTTGTGCGTACCGATCAGAATATCGATTTTCCCTTCCGCCACTccc                                                                            >  1:266963/1‑90 (MQ=255)                   ttAAACTTGACGTCACTTTGCAGCAGTTTGTGCGTACCGATCAGAATATCGATTTTCCCTTCCGCCACTCCCGCAAGGATTTGCGTCTGc                                                          <  2:581153/90‑1 (MQ=255)                   ttAAACTTGACGTCACTTCGCAGCAGTTTGGGGGTACCGATCAGAATATCGTTTTTCCCTTCCGCCACTCCCGCAAGGATTTGCGTCTGc                                                          <  1:724287/90‑1 (MQ=255)                         ttGACGTCACTTTGCAGCAGTTTGTGCGTACCGATCAGAATATCGATTTTCCCTTACGCCACTCCCGCAAGGATTTGCGTCTGCTCTTTg                                                    >  1:387189/1‑90 (MQ=255)                           gACGTCACTTTGCAGCAGTTTGTGCGTACCGATCAGAATATCGATTTTCCCTTCCGCCACTCCCGCAAGGATTTGCGTCTGCTCTTTGgc                                                  >  2:167184/1‑90 (MQ=255)                                    ttGCAGCAGTTTGTGCGTACCGATCAGAATATCGATTTTCCCTTCCGCCACTCCCGCAAGGATTTGCGTCTGCTCTTTGGCGCTGCGGaa                                         <  2:264833/90‑1 (MQ=255)                                           agTTTGTGCGTACCGATCAGAATATCGATTTTCCCTTCCGCCACTCCCGCAAGGATTTGCGTCTGCTCTTTGGCGCTGCGGAAACGGgag                                  <  2:406581/90‑1 (MQ=255)                                                  gCGTACCCAACAGAATATCGATTTCCCCTCCCGCCCCTCCCGCAAGGATTTGCGTCTGCTCTTTGGCGCTGCGGAAACGGGAGATCAttt                           <  1:131698/90‑1 (MQ=255)                                                         gATCAGAATATCGATTTTCCCTTCCGCCACTCCCGCAAGGATTTGCGTCTGCTCTTTGGCGCTGCGGAAACGGGAGATCATTTCGATACg                    >  1:248153/1‑90 (MQ=255)                                                          aTCAGAATATCGATTTTCCCTTCCGCCACTCCCGCAAGGATTTGCGTCTGCTCTTTGGCGCTGCGGAAACGGGAGATCATTTCGATACGt                   >  2:663537/1‑90 (MQ=255)                                                                        tttCCCTTCCGCCACTCCCGCAAGGATTTGCGTCTGCTCTTTGGCGCTGCGGAAACGGGAGATCATTTCGATACGTACCGGCCAGTTGGc     >  2:626197/1‑90 (MQ=255)                                                                           cccTTCCGCCACTCCCGCAAGGATTTGCGTCTGCTCTTTGGCGCTGCGGAAACGGGAGATCATTTCGATACGTACCGGCCAGTTGGCGaa  >  2:409598/1‑90 (MQ=255)                                                                                        |                                                                             ATCAGCAGGCCTAAATCTTTAAACTTGACGTCACTTTGCAGCAGTTTGTGCGTACCGATCAGAATATCGATTTTCCCTTCCGCCACTTCCGCAAGGATTTGCGTCTGCTCTTTGGCGCTGCGGAAACGGGAGATCATTTCGATACGTACCGGCCAGTTGGCGAA  >  NZ_CP009273/1167241‑1167404

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑