| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,335,990 | A→T | intergenic (+6/‑188) | lapB → / → pyrF | lipopolysaccharide assembly protein LapB/orotidine‑5'‑phosphate decarboxylase |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,335,990 | 0 | A | T | 81.2% | 36.3 / 4.2 | 16 | intergenic (+6/‑188) | lapB/pyrF | lipopolysaccharide assembly protein LapB/orotidine‑5'‑phosphate decarboxylase |
| Reads supporting (aligned to +/- strand): ref base A (1/2); new base T (7/6); total (8/8) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.00e+00 | |||||||||||
ATACACCCTCTACTGGCATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTTAAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCAACGAC > NZ_CP009273/1335906‑1336054 | aTACACCCTCTACTGGCATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTTtaaaaa > 2:685912/1‑90 (MQ=255) cccTCTACTGGCATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTTAAAAAAAATcc > 1:327978/1‑90 (MQ=255) ctACTGGCATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTTTAAAAAAATCCGACt > 1:643508/1‑90 (MQ=255) ctACTGGCATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTTTAAAAAAATCCGACt > 2:355072/1‑90 (MQ=255) ggCATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTTTAAAAAAATCCGACTTTAGt < 2:556751/90‑1 (MQ=255) gCATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTTTAAAAAAATCCGACTTTAGtt > 1:389416/1‑90 (MQ=255) aTTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTTTAAAAAAATCCGACTTTAGTTac < 1:611220/90‑1 (MQ=255) gTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTTTAAAAAAATCCGACTTTAGTTACAACATAc < 2:626496/90‑1 (MQ=255) gTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTTAAAAAAAATCCGACTTTAGTTACAACATAc < 2:327978/90‑1 (MQ=255) cTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTTTAAAAAAATCCGACTTTAGTTACAACATACTa < 1:189757/90‑1 (MQ=255) aaCCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTTTAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCAt < 2:449791/90‑1 (MQ=255) aCCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTTTAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCAtt > 2:529660/1‑90 (MQ=255) tAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTTTAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCt > 2:605748/1‑90 (MQ=255) gATTCGCGGTCTTGATGGCCTGTAATTTTTTAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCa > 2:465469/1‑90 (MQ=255) gcgGTCTTGATGGCCTGTAATTTTTAAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCAacgac < 2:621541/90‑1 (MQ=255) tGTAATTTTTTAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCAacgac < 1:417900/75‑1 (MQ=255) tGTAATTTTTTAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCAacgac > 2:417900/1‑75 (MQ=255) | ATACACCCTCTACTGGCATTGTCCGTCTTGTCGGGCCTGGTCAACCATTAAACCGATTCGCGGTCTTGATGGCCTGTAATTTTTAAAAAAAATCCGACTTTAGTTACAACATACTAATTATTAATGTTCCATTGTGCTCCGGCAACGAC > NZ_CP009273/1335906‑1336054 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |