| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 115,628 | A→C | T137P (ACG→CCG) | ampD → | 1,6‑anhydro‑N‑acetylmuramyl‑L‑alanine amidase AmpD |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 115,628 | 0 | A | C | 97.1% | 99.8 / NA | 35 | T137P (ACG→CCG) | ampD | 1,6‑anhydro‑N‑acetylmuramyl‑L‑alanine amidase AmpD |
| Reads supporting (aligned to +/- strand): ref base A (0/0); major base C (14/20); minor base T (1/0); total (15/20) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 4.29e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.85e-01 | |||||||||||
CAATGATTTTTCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCC > NZ_CP009273/115543‑115713 | cAATGATTTTTCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCcgcg < 1:52265/90‑1 (MQ=255) tGATTTTTCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGc < 1:394692/90‑1 (MQ=255) tGATTTTTCTAGCGGGTTTGACCTTGAAGGAACCGGTACGCTGGCTTTTCCCGATGCGCAGTATCAACAGCTTGCTGCACTTCCGCGGGc < 1:279993/90‑1 (MQ=255) tttttCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACt < 2:133186/90‑1 (MQ=255) ttttCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACtt > 2:729295/1‑89 (MQ=255) ttttCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACtg > 1:680756/1‑90 (MQ=255) tCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACtgatt < 2:680756/90‑1 (MQ=255) ttGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGc > 2:570362/1‑79 (MQ=255) ttGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGc < 1:570362/79‑1 (MQ=255) gCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTa < 1:185071/90‑1 (MQ=255) gCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTa < 2:103031/90‑1 (MQ=255) cACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCgggg > 2:642214/1‑59 (MQ=255) cACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGc < 1:642214/60‑1 (MQ=255) cGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGa < 1:390360/90‑1 (MQ=255) tGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACgg < 2:467167/90‑1 (MQ=255) ggCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACggg < 1:163025/90‑1 (MQ=255) ggCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACggg < 2:347750/90‑1 (MQ=255) gCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGc < 1:138154/90‑1 (MQ=255) cGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGcc < 2:441161/90‑1 (MQ=255) cGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGcc < 2:711981/90‑1 (MQ=255) tataCCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCAt < 2:625602/90‑1 (MQ=255) ataCCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGGCAtt > 1:184110/1‑90 (MQ=255) taCCGATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGGACTGATTTATTGCTATCCCGGTAACGCTAAAAACATGACGGGCCATtg > 1:525183/1‑90 (MQ=255) gATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGGACTGATTGATTGCTATCCGGATATCGCTAAAAAAATGACGGGGCCTTGTGat > 1:655789/1‑90 (MQ=255) gATGCGCAGTATCAACAGCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGat > 2:501859/1‑90 (MQ=255) gATGCGCAGTATCAACAGCTTGCGGCGGGTTCGCGGGGGCTGATTTATTCCTATCCGGAAATCGCTAAAAACATGACGGGCCATTGTGat > 1:154780/1‑90 (MQ=255) gCTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGaa < 2:261548/90‑1 (MQ=255) cTTGCGGCGGTTCCGCGGGCACTGTTTGATTGCTATCCGGGTATCGCTAAAAACATGAGGGGCCATTGTGATATTGCGCCGGATCGGgaa > 1:152484/1‑90 (MQ=255) cTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGGTATCGCTAAAAACATGACGGGCCATTGTGATATTTCGCCGGATCGGaaa > 2:610992/1‑90 (MQ=255) cTTGCGGCGGTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGGGATATTGCGCCGGATCGGaaa > 2:768849/1‑90 (MQ=255) cTTGCGGCGGTTCCCCGGGGACTGATTGATTTCTATCCGGATAACGCTAAAAACATGACGGGCCATTGTGATATTGCGCCCGATCGGaaa > 1:652939/1‑90 (MQ=255) cTTGCGGCGGGTCCGCGGGCACTGATTGATTGCTATCCGGATATTGCTAAAAACATTAAGGGGCCTTGTGGGATTTCGCCGGGTCGGaaa > 2:179438/1‑90 (MQ=255) gcggTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCgg > 2:666678/1‑76 (MQ=255) gcggTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCgg < 1:666678/76‑1 (MQ=255) ggTTCCGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATcc < 1:235911/90‑1 (MQ=255) | CAATGATTTTTCTATTGGGATTGAGCTTGAAGGCACCGATACGCTGGCGTATACCGATGCGCAGTATCAACAGCTTGCGGCGGTTACGCGGGCACTGATTGATTGCTATCCGGATATCGCTAAAAACATGACGGGCCATTGTGATATTGCGCCGGATCGGAAAACCGATCC > NZ_CP009273/115543‑115713 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 10 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |