Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,394,163	G→A	L111L (CTG→TTG)	ogt ←	methylated‑DNA‑‑[protein]‑cysteine S‑methyltransferase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,394,163	0	G	A	100.0%	98.8 / NA	26	L111L (CTG→TTG)	ogt	methylated‑DNA‑‑[protein]‑cysteine S‑methyltransferase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base A (7/19);  total (7/19)

GGCAAGGTACGACGATGCTGATGGGATTCGATCCGTTTGCCGCACCAACGGCACGCGC‑‑CGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCGTAG  >  NZ_CP009273/1394076‑1394246                                                                                          |                                                                                    ggCAAGGTACGACGATGCTGATGGGATTCGATCCGTTTGCCGCACCAACGGCCCGCGC‑‑CGCGCCAGGACGGCCCAATTGCTCAGCCAAtt                                                                                   <  2:477527/90‑1 (MQ=255)     aGGTACGACGATGCTGATGGGATTCGATCCGTTTGCCGCACCAACGGCACGCGC‑‑CGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGcc                                                                               >  1:110122/1‑90 (MQ=255)       gTACGACGATGCTGATGGGATTCGATCCGTTTGCCGCACCAACGGCACGCGC‑‑CGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGt                                                                             <  1:476178/90‑1 (MQ=255)               aTGCTGATGGGATTCGATCCGTTTGCCGCACCAACGGCACGCGC‑‑CGCGCCAGGACGGCCCAATTGCTCAGCCAAtt                                                                                   >  2:696182/1‑76 (MQ=255)               aTGCTGATGGGATTCGATCCGTTTGCCGCACCAACGGCACGCGC‑‑CGCGCCAGGACGGCCCAATTGCTCAGCCAAtt                                                                                   <  1:696182/76‑1 (MQ=255)                 gCTGATGGGATTCGATCCGTTTGCCGCACCAACGGCACGCGC‑‑CGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGc                                                                                >  1:186855/1‑77 (MQ=255)                 gCTGATGGGATTCGATCCGTTTGCCGCACCAACGGCACGCGC‑‑CGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGc                                                                                <  2:186855/77‑1 (MQ=255)                  cTGATGGGATTCGATCCGTTTGCCGCACCAACGGCACGCGC‑‑CGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCATTAcc                                                                  <  1:25034/90‑1 (MQ=255)                  cTGATGGGATTCGATCCGTTTGCCGCACCAACGGCACGCGC‑‑CGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCATTAcc                                                                  <  1:364585/90‑1 (MQ=255)                      tGGGATTCGATCCGTTTGCCGCACCAACGGCACGCGC‑‑CGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGcc                                                              >  2:297228/1‑90 (MQ=255)                        ggATTCGATCCGTTTGCCGCACCAACGGCACGCGC‑‑CGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCg                                                            <  2:589951/90‑1 (MQ=255)                          aTTCGATCCGTTTGCCGCACCAACGGCACGCGCCGCGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCg                                                            <  2:630527/90‑1 (MQ=255)                          aTTCGATCCGTTTGCCGCACCAACGGCACGCGC‑‑CGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCa                                                          <  1:233450/90‑1 (MQ=255)                           ttCGATCCGTTTGCCGCACCAACGGCACGCGC‑‑CGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAg                                                         <  2:74656/90‑1 (MQ=255)                                  cGTTTGCCGCCCCAACGGCACGCGC‑‑CGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAGGGGATAg                                                  <  2:674522/90‑1 (MQ=255)                                  cGTTTGCCGCACCAACGGCACGCGC‑‑CGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAGGGGATAg                                                  <  2:738583/90‑1 (MQ=255)                                       gCCGCACCAACGGCACGCGC‑‑CGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGt                                             <  2:605333/90‑1 (MQ=255)                                           cACCAACGGCACGCGC‑‑CGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAgtg                                         <  1:685969/90‑1 (MQ=255)                                           cACCAACGGCACGCGC‑‑CGCGCCAGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAgtg                                         <  2:68234/90‑1 (MQ=255)                                                       gcgc‑‑cgcgccAGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACtt                             >  2:469512/1‑90 (MQ=255)                                                        cgc‑‑cgcgccAGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTc                            <  1:297228/90‑1 (MQ=255)                                                          c‑‑cgcgccAGGACGGCCCAATTGCTCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTcgc                          <  1:729108/90‑1 (MQ=255)                                                                                   tCAGCCAATTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCGTa   >  2:580226/1‑90 (MQ=255)                                                                                    cAGCCAATTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCGTAg  <  2:56264/90‑1 (MQ=255)                                                                                      gCCAATTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCGTAg  <  2:293798/88‑1 (MQ=255)                                                                                      gCCAATTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCGTAg  >  1:293798/1‑88 (MQ=255)                                                                                          |                                                                                    GGCAAGGTACGACGATGCTGATGGGATTCGATCCGTTTGCCGCACCAACGGCACGCGC‑‑CGCGCCAGGACGGCCCAATTGCTCAGCCAGTTGGCCGTAATGCATTACCTGCCCGCAGGGGATAGTGCGTAGTGTTTTCCAGACTTCGCGCTGAAATGGCGTCCCCCCCGTAG  >  NZ_CP009273/1394076‑1394246

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 22 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑