Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,394,663	T→C	G456G (GGA→GGG)	abgT ←	p‑aminobenzoyl‑glutamate transporter

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,394,663	0	T	C	100.0%	72.6 / NA	22	G456G (GGA→GGG)	abgT	p‑aminobenzoyl‑glutamate transporter
Reads supporting (aligned to +/- strand):  ref base T (0/0);  new base C (13/9);  total (13/9)

CCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCAATACGAAAGAGGATT  >  NZ_CP009273/1394581‑1394738                                                                                   |                                                                            ccaccaAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGaa                                                                      <  1:393732/90‑1 (MQ=255)   accaAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAAc                                                                    <  2:726710/90‑1 (MQ=255)              aaGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACaaa                                                         <  2:196399/90‑1 (MQ=255)                     tAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGAt                                                  >  1:303/1‑90 (MQ=255)                       gggCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATAc                                                >  1:444745/1‑90 (MQ=255)                         gCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCg                                              <  2:44157/90‑1 (MQ=255)                         gCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCg                                              >  2:293862/1‑90 (MQ=255)                          cAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCgg                                             >  1:147790/1‑90 (MQ=255)                          cAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCgg                                             >  1:696789/1‑90 (MQ=255)                          cAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCgg                                             <  1:529434/90‑1 (MQ=255)                          cAAGACTAACCAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCgg                                             <  1:72457/90‑1 (MQ=255)                               ataccGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTa                                        <  1:390837/86‑1 (MQ=255)                                 aaCGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTaaa                                      >  1:745482/1‑90 (MQ=255)                                  aCGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAg                                     <  1:128203/90‑1 (MQ=255)                                           aagtaCCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACgg                            <  1:3017/90‑1 (MQ=255)                                            agtaCCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGa                           >  2:523426/1‑90 (MQ=255)                                             gtaCCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGAt                          >  2:694995/1‑90 (MQ=255)                                              taCCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATg                         >  2:462909/1‑90 (MQ=255)                                               aCCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGa                        >  2:296503/1‑90 (MQ=255)                                               aCCCAGTTTCGCGTCTGGGTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGa                        >  2:695296/1‑90 (MQ=255)                                                    gTTTCGCGTCTGGTTTGTAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCgg                   >  2:169190/1‑90 (MQ=255)                                                                     tAGCGTTGCAGGAACCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCAATACGAAAGAGGAtt  >  1:390574/1‑90 (MQ=255)                                                                                   |                                                                            CCACCAAAAAGATAAGTGGATAGGGCAAGACTAACGAATAGTAAGTACCCAGTTTCGCGTCTGGTTTGTAGCGTTGCAGGAATCCAAGAAACAGTGGAACAAAAGGAGATACCGGCGCTAAAGGCAATACGGATGAGTCGGCAATACGAAAGAGGATT  >  NZ_CP009273/1394581‑1394738

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑