Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,476,692	G→C	G142G (GGC→GGG)	azoR ←	FMN‑dependent NADH‑azoreductase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,476,692	0	G	C	100.0%	88.1 / NA	26	G142G (GGC→GGG)	azoR	FMN‑dependent NADH‑azoreductase
Reads supporting (aligned to +/- strand):  ref base G (0/0);  new base C (11/15);  total (11/15)

ATTTCACATCGGTAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGC  >  NZ_CP009273/1476607‑1476777                                                                                      |                                                                                      aTTTCACATCGGTAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCccccgc                                                                                   <  2:679130/90‑1 (MQ=255)     cacaTCGGTAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCCCCGCGGCt                                                                               <  1:540667/90‑1 (MQ=255)     cacaTCGGTAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCCCCGCGGCt                                                                               <  2:551542/90‑1 (MQ=255)           ggTAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCCCCGCGGCTGGtaat                                                                         >  2:625095/1‑90 (MQ=255)            gTAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCCCCAGGTCCGTTGGTCCATCTTTGGGGATCCCCCCGCGGCTGGtaata                                                                        >  1:214288/1‑90 (MQ=255)                   cGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCCCCGCGGCTGGTAATAACGATgg                                                                 >  1:252923/1‑90 (MQ=255)                    gATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCCCCGCGGCTGGTAATAACGATGGc                                                                >  2:439769/1‑90 (MQ=255)                    gATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCCCCGCGGCTGGTAATAACGATGGc                                                                >  2:526180/1‑90 (MQ=255)                              aGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCCCCGCGGCTGGTAATAACGATGGCTTTTTTAccc                                                      <  1:484043/90‑1 (MQ=255)                               ggAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCCCCGCGGCTGGTAATAACGATGGCTTTTTTACCCg                                                     <  1:371450/90‑1 (MQ=255)                               ggAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCCCCGCGGCTGGTAATAACGATGGCTTTTTTACCCg                                                     <  2:122516/90‑1 (MQ=255)                               ggAACGTAGAGAGATACGGCGTCACCAGGTCCGTTTGTCCATCTTTGTGGAGGCCCCCGTGGCTGGTAATAACGATGGCTTCTTTACCCg                                                     <  1:149732/90‑1 (MQ=255)                                       gACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCCCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGa                                             <  2:457257/90‑1 (MQ=255)                                                 gCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCCCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCGGAc                                   <  2:431765/90‑1 (MQ=255)                                                   gTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCCCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCGGACCg                                 >  1:691628/1‑90 (MQ=255)                                                   gTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCCCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCGGACCg                                 <  2:441129/90‑1 (MQ=255)                                                   gTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCCCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCGGACCg                                 <  1:196122/90‑1 (MQ=255)                                                     cACCAGGTCCGTTGGTCCATCTTTGTGGATCCCCCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCGGACCGtt                               >  1:531206/1‑90 (MQ=255)                                                            tCCGTTGGTCCATCTTTGTGGATCCCCCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCGGACCGTTCTCGGta                        <  1:624661/90‑1 (MQ=255)                                                                   gTCCATCTTTGTGGATCCCCCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCGGACCGTTCTCGGTATAGCGGa                 <  2:278614/90‑1 (MQ=255)                                                                     ccATCTTTGTGGATCCCCCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCGGACCGTTCTCGGTATAGCgg                  <  2:548817/87‑1 (MQ=255)                                                                     ccATCTTTGTGGATCCCCCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCGGACCGTTCTCGGTATAGCgg                  >  1:548817/1‑87 (MQ=255)                                                                             gtgGATCCCCCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGcc       >  2:240768/1‑90 (MQ=255)                                                                                 aTCCCCCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCGGACAGTTCTCGGTATAGCGGAAAGTAACGCCTgcg   <  2:70212/90‑1 (MQ=255)                                                                                  tCCCCCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTgcgc  <  2:324108/90‑1 (MQ=255)                                                                                  tCCCCCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTgcgc  >  2:287122/1‑90 (MQ=255)                                                                                  tCCCCCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTgcgc  >  2:701765/1‑90 (MQ=255)                                                                                      |                                                                                      ATTTCACATCGGTAATGCCGATAAAGCCGAGGAACGTGGACAGATACGGCGTCACCAGGTCCGTTGGTCCATCTTTGTGGATCCCGCCGCGGCTGGTAATAACGATGGCTTTTTTACCCGTTACCAGACCTTCCGGACCGTTCTCGGTATAGCGGAAAGTAACGCCTGCGC  >  NZ_CP009273/1476607‑1476777

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 21 ≤ ATCG/ATCG < 30 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑