| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 1,494,123 | T→C | E195G (GAA→GGA) | ydcK ← | YdcK family protein |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 1,494,123 | 0 | T | C | 96.8% | 105.0 / ‑3.6 | 31 | E195G (GAA→GGA) | ydcK | YdcK family protein |
| Reads supporting (aligned to +/- strand): ref base T (0/0); major base C (16/14); minor base G (1/0); total (17/14) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.00e+00 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 9.98e-01 | |||||||||||
ATCTTCTTCTATGCCAGCTTTCACCTGCGCGTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTTCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCTGGTG > NZ_CP009273/1494038‑1494208 | aTCTTCTTCTATGCCAGCTTTCACCTGCGCGTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAAt > 1:598613/1‑90 (MQ=255) cttcttctATGCCAGCTTTCACCTGCGCGTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATAc < 2:261050/90‑1 (MQ=255) ttcttctATGCCAGCTTTCACCTGCGCGTGACCGTAGACGTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACt > 2:587452/1‑90 (MQ=255) cTTTCACCTGCGCGTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCaaaa > 2:557076/1‑89 (MQ=255) cTTTCACCTGCGCGTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCaaaa < 1:557076/89‑1 (MQ=255) gcgcGTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGgcgc < 1:627457/90‑1 (MQ=255) gcgTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGa < 2:252613/90‑1 (MQ=255) gcgTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGa < 1:587452/90‑1 (MQ=255) tGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGt < 1:537094/90‑1 (MQ=255) gACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGtt < 1:4132/90‑1 (MQ=255) aCCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATATTGGCAAAATCAAAAACTTCGGCGCGATGTTc < 2:748390/90‑1 (MQ=255) gTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAAt < 1:670108/90‑1 (MQ=255) gTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAAt > 2:670108/1‑90 (MQ=255) ttAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCa > 2:489902/1‑90 (MQ=255) gcgcAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAAt > 1:423967/1‑80 (MQ=255) gcgcAATCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAAt < 2:423967/80‑1 (MQ=255) aTCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGgac > 2:341421/1‑90 (MQ=255) aTCGCATAGCCAGACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGgac > 1:271622/1‑90 (MQ=255) gACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAAt > 2:127968/1‑63 (MQ=255) gACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAAt < 1:556806/63‑1 (MQ=255) gACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAAt > 2:556806/1‑63 (MQ=255) gACATTGTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAAt < 1:127968/63‑1 (MQ=255) gTTCTCTTCATTACCTGCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGGCGACGGCATCGCCATAAAt > 1:402239/1‑90 (MQ=255) gTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCAt > 2:194298/1‑65 (MQ=255) gTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCAt < 1:194298/65‑1 (MQ=255) gTTCTCTTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAAt > 1:768862/1‑90 (MQ=255) ctTCATTACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTtgtg > 1:236687/1‑90 (MQ=255) ttACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCtgg > 2:200350/1‑90 (MQ=255) ttACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCtgg > 2:398171/1‑90 (MQ=255) ttACCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCtgg > 1:104200/1‑90 (MQ=255) aCCTCCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCtggtg < 1:235956/90‑1 (MQ=255) | ATCTTCTTCTATGCCAGCTTTCACCTGCGCGTGACCGTAGACTTTAGCGCAATCGCATAGCCAGACATTGTTCTCTTCATTACCTTCAATACTGGCAAAATCAAAAACTTCGGCGCGATGTTCAATAAAAGCATACCGGACGACGGCATCGCCATAAATTTGTGCCTGGTG > NZ_CP009273/1494038‑1494208 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 27 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |