| Predicted mutation | ||||||
|---|---|---|---|---|---|---|
| evidence | seq id | position | mutation | annotation | gene | description |
| RA | NZ_CP009273 | 118,882 | G→T | E102* (GAA→TAA) | pdhR → | pyruvate dehydrogenase complex transcriptional repressor PdhR |
| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 118,882 | 0 | G | T | 84.0% | 62.3 / 8.5 | 25 | E102* (GAA→TAA) | pdhR | pyruvate dehydrogenase complex transcriptional repressor PdhR |
| Reads supporting (aligned to +/- strand): ref base G (1/3); new base T (9/12); total (10/15) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 6.26e-01 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.72e-01 | |||||||||||
TTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATGACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCAC > NZ_CP009273/118794‑118971 | ttttGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATGACTTGCTCGa < 1:756090/90‑1 (MQ=255) cAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATGACTTGCTCTAAacacga < 1:125353/90‑1 (MQ=255) gagCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATGACTTGCTCTAAacacgaca > 2:253951/1‑90 (MQ=255) gagCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATGACTTGCTCTAAacacgaca < 1:762709/90‑1 (MQ=255) agcagcCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATGACTTGCTCTAAacacgacac < 2:739912/90‑1 (MQ=255) agcagcCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATGACTTGCTCTAAacacgacac < 2:521059/90‑1 (MQ=255) agcagcCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATGACTTGCTCTAAacacgacac < 1:194230/90‑1 (MQ=255) gcagcCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATGACTTGCTCTAAacacgacacg < 2:511362/90‑1 (MQ=255) gcagcCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATGACTTGCTCTAAacacgacacg < 2:337201/90‑1 (MQ=255) gcCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATGACTTGCTCTAAACACGACACGccc > 2:608134/1‑90 (MQ=255) tATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATGACTTGCTCTAAACACGACACGCCCTgg < 1:253951/90‑1 (MQ=255) tGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATGACTTGCTCTAAACACGACACGCCCTGGaa > 2:119404/1‑90 (MQ=255) tGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATGACTTGCTCTAAACACGACACGCCCTGGaa > 1:110679/1‑90 (MQ=255) cTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATGACTTGCTCTAAACACGACACGCCCTGGAAGGTATcgc < 2:101622/90‑1 (MQ=255) tCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATGACTTGCTCTAAACACGACACGCCCTGGAAGGTATcgccg < 2:330263/90‑1 (MQ=255) tCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATGACTTGCTCTAAACACGACACGCCCTGGAAGGTATCGCCGCttatta > 1:232078/1‑90 (MQ=255) tggAGCTGCTCTCCGACCATCCTGAGTCACAGTATGACTTGCTCTAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCgcgc < 2:232078/90‑1 (MQ=255) tggAGCTGCTCTCCGACCATCCTGAGTCACAGTATGACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCgcgc < 2:277554/90‑1 (MQ=255) tggAGCTGCTCTCCGACCATCCTGAGTCACAGTATGACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCgcgc < 2:277555/90‑1 (MQ=255) ctcCGACCATCCTGAGTCACAGTATGACTTGCTCTAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTAc < 1:148576/90‑1 (MQ=255) aTCCTGAGTCACAGTATGACTTGCTCTAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAg > 2:679044/1‑90 (MQ=255) caGTATGACTTGCTCTAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGc > 1:420671/1‑90 (MQ=255) caGTATGACTTGCTCTAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGc > 1:139908/1‑90 (MQ=255) tGCTCTAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAAc > 2:193322/1‑90 (MQ=255) tGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAAc > 2:602126/1‑90 (MQ=255) gAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTccac < 1:123649/90‑1 (MQ=255) | TTTTGTCCAGAGCAGCCTATGGCAAAGCTTCAGCGATCCGCTGGTGGAGCTGCTCTCCGACCATCCTGAGTCACAGTATGACTTGCTCGAAACACGACACGCCCTGGAAGGTATCGCCGCTTATTACGCCGCGCTGCGTAGTACCGATGAAGACAAGGAACGCATCCGTGAACTCCAC > NZ_CP009273/118794‑118971 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 29 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |