Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,547,935	A→G	V54A (GTA→GCA)	adhP ←	alcohol dehydrogenase AdhP

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,547,935	0	A	G	100.0%	99.7 / NA	29	V54A (GTA→GCA)	adhP	alcohol dehydrogenase AdhP
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (16/13);  total (16/13)

GCACGATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACTCCATTTTCAGCAGGG  >  NZ_CP009273/1547857‑1548016                                                                               |                                                                                  gCACGATCGCCTGGTTTTAATGAGGGGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTg                                                                        >  2:2428/1‑90 (MQ=255)     gATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCAc                                                                    >  1:333687/1‑90 (MQ=255)               ttttAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGcc                                                          <  1:561329/90‑1 (MQ=255)                tttAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCa                                                         >  1:153591/1‑90 (MQ=255)                tttAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCa                                                         >  1:535831/1‑90 (MQ=255)                           gACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATg                                              >  1:93087/1‑90 (MQ=255)                            acacCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGa                                             <  1:2428/90‑1 (MQ=255)                                 tGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATc                                        <  1:602721/90‑1 (MQ=255)                                  ggACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCg                                       >  1:207275/1‑90 (MQ=255)                                     cccACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTa                                    <  1:277051/90‑1 (MQ=255)                                      ccACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTAt                                   <  2:358969/90‑1 (MQ=255)                                       cACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCaaa                                                                <  1:163896/60‑1 (MQ=255)                                       cACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCaaa                                                                >  2:163896/1‑60 (MQ=255)                                       cACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATg                                  >  2:659219/1‑90 (MQ=255)                                         cTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGAc                                >  2:210079/1‑90 (MQ=255)                                           tCTGCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACAt                              <  2:515168/90‑1 (MQ=255)                                              gCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGc                                                           <  2:428120/58‑1 (MQ=255)                                              gCCACCACACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGc                                                           >  1:428120/1‑58 (MQ=255)                                                  ccacACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATAcaccac                       >  1:697370/1‑90 (MQ=255)                                                  ccacACCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATAcaccac                       <  1:584629/90‑1 (MQ=255)                                                   cacaCCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTa                                    >  1:64964/1‑76 (MQ=255)                                                   cacaCCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTa                                    <  2:64964/76‑1 (MQ=255)                                                      acCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCacaaca                   >  1:491462/1‑90 (MQ=255)                                                      acCGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCacaaca                   <  2:614961/90‑1 (MQ=255)                                                        cGATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACt                 <  2:93087/90‑1 (MQ=255)                                                         gATGCCTTCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACTc                <  2:368712/90‑1 (MQ=255)                                                                tCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACTCCATTTTc         >  2:209552/1‑90 (MQ=255)                                                                tCATGGCCCAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACTCCATTTTc         >  2:394248/1‑90 (MQ=255)                                                                       ccAGAATTGCGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACTCCATTTTCAGCAggg  >  1:37689/1‑90 (MQ=255)                                                                               |                                                                                  GCACGATCGCCTGGTTTTAATGAGGTGACACCTGGACCCACTTCTGCCACCACACCGATGCCTTCATGGCCCAGAATTACGCCGGTTTTGTCACCAAAATCGCCATTCTTAACATGAAGATCGGTATGACATACACCACAACACTCCATTTTCAGCAGGG  >  NZ_CP009273/1547857‑1548016

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 24 ≤ ATCG/ATCG < 31 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑