Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	1,558,439	A→G	Y518H (TAT→CAT)	dosP ←	oxygen‑sensing cyclic‑di‑GMP phosphodiesterase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	1,558,439	0	A	G	100.0%	103.9 / NA	30	Y518H (TAT→CAT)	dosP	oxygen‑sensing cyclic‑di‑GMP phosphodiesterase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base G (13/17);  total (13/17)

TGCGCCTAAAACC‑AAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCTGATGCCAATACTCAAGGTAAG  >  NZ_CP009273/1558353‑1558520                                                                                        |                                                                                  cgcgcCAAAACCC‑AACCCCTTTTTTCCCTTTTCGTTCATCCCCGGGCTAAAAACCTCCCAGCCGTTACCGCCATTCTTGCGAATATGATc                                                                                <  1:556460/89‑1 (MQ=255)  gcgcCTAAAACC‑AAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATcc                                                                               >  2:478791/1‑90 (MQ=255)  gcgcCTAAAACC‑AAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATcc                                                                               >  1:48268/1‑90 (MQ=255)       caaaaCCAAAACGCTTTTTTCCCTTTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCcattg                                                                           <  2:164391/89‑1 (MQ=255)           aCC‑AACCGCTTTTTTCCCTTTTCTTTCATCGCGGGGTTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCcattgcattg                                                                      <  1:644736/90‑1 (MQ=255)           aCC‑AAACGCTCTTTTCCCTTTTCGTTCCTCCCCGGGCTGAAAAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCcattgcattg                                                                      <  2:201079/90‑1 (MQ=255)            acaaaaCGCTTTTTTCCCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCcattgcattg                                                                      <  2:315263/87‑1 (MQ=255)                 ccGCTTTTTTCCCATTTCTTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGc                                                                 <  2:130482/89‑1 (MQ=255)                     tctTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTg                                                             >  1:125499/1‑90 (MQ=255)                     tctTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTg                                                             >  1:476109/1‑90 (MQ=255)                       ttttACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGga                                                           >  1:749612/1‑90 (MQ=255)                                ttCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTa                                                  >  1:499939/1‑90 (MQ=255)                                ttCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTa                                                  >  2:153096/1‑90 (MQ=255)                                   gTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATc                                               >  1:492704/1‑90 (MQ=255)                                     tCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATcgc                                             >  1:422094/1‑90 (MQ=255)                                      caccgcgGGGCTGAAGACCTCCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATcgcg                                            <  2:672975/87‑1 (MQ=255)                                      caccGCCGGGCTGAAGACCGGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATcgcg                                            <  2:200517/87‑1 (MQ=255)                                      cATCGCGGGGCTGAAAACCTCCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATcgcg                                            <  1:308226/90‑1 (MQ=255)                                      cATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATcgcg                                            <  2:476109/90‑1 (MQ=255)                                        tCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGt                                          >  2:129517/1‑90 (MQ=255)                                           ccGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGtttt                                       >  1:11796/1‑90 (MQ=255)                                                cTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTAccc                                  <  2:749612/90‑1 (MQ=255)                                                         tGCCAGCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAg                         >  2:773073/1‑90 (MQ=255)                                                            cAGCGGTTACCGCCATTCTTGCGAATATGATCCATTGCGTTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCTg                      <  1:224583/90‑1 (MQ=255)                                                              gCCGTTACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCTGAt                    <  2:422094/90‑1 (MQ=255)                                                                  ttACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCTGATGCCa                <  2:492704/90‑1 (MQ=255)                                                                  ttACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCTGATGCCa                <  2:11796/90‑1 (MQ=255)                                                                   tACCGCCATTCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCTGATGCCaa               <  2:524487/90‑1 (MQ=255)                                                                            tCTTGCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCTGATGCCAATACTCAAgg      >  1:380588/1‑90 (MQ=255)                                                                                gCGAATATGATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCTGATGCCAATACTCAAGGTAAg  <  2:283992/90‑1 (MQ=255)                                                                                        |                                                                                  TGCGCCTAAAACC‑AAACGCTCTTTTACCATTTCGTTCATCGCCGGGCTGAAGAACTGCCAGCCGTTACCGCCATTCTTGCGAATATAATCCATTGCATTGTGAGCAGTGGAGAGCAAGTAATCGCGGTTTTTACCCAGGTCGTAGCTGATGCCAATACTCAAGGTAAG  >  NZ_CP009273/1558353‑1558520

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 34 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑