Predicted mutation
evidence	seq id	position	mutation	annotation	gene	description
RA	NZ_CP009273	130,698	A→T	*866L (TAA→TTA)	acnB →	bifunctional aconitate hydratase 2/2‑methylisocitrate dehydratase

Read alignment evidence...
	seq id	position		ref	new	freq	score (cons/poly)	reads	annotation	genes	product
*	NZ_CP009273	130,698	0	A	T	100.0%	79.7 / NA	21	*866L (TAA→TTA)	acnB	bifunctional aconitate hydratase 2/2‑methylisocitrate dehydratase
Reads supporting (aligned to +/- strand):  ref base A (0/0);  new base T (18/3);  total (18/3)

CTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCAT  >  NZ_CP009273/130620‑130780                                                                               |                                                                                   cTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAgcg                                                                         >  2:338462/1‑90 (MQ=255)         tATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCt                                                                 >  1:716433/1‑90 (MQ=255)         tATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCt                                                                 >  2:701387/1‑90 (MQ=255)         tATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCt                                                                 >  2:390099/1‑90 (MQ=255)         tATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCt                                                                 >  1:102037/1‑90 (MQ=255)          aTCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTg                                                                <  2:318809/90‑1 (MQ=255)                        cAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGacac                                                  >  1:753819/1‑90 (MQ=255)                          gCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACaa                                                >  2:278653/1‑90 (MQ=255)                               ctcAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGccc                                           <  1:338462/90‑1 (MQ=255)                                tcAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCg                                          <  1:501437/90‑1 (MQ=255)                                        ccGCGAAAGCCGATGGGGGGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATga                                  >  1:390302/1‑90 (MQ=255)                                        ccGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATga                                  >  2:400377/1‑90 (MQ=255)                                             aaaGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTc                             >  1:73899/1‑90 (MQ=255)                                             aaaGCCGATGGGGTGATTTTCCAGACCGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTc                             >  2:565438/1‑90 (MQ=255)                                               aGCCGATGGGGTGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTccc                           >  1:447299/1‑90 (MQ=255)                                                          tGATTTTCCAGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCAtttttt                >  2:124872/1‑90 (MQ=255)                                                                   aGACTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTaa       >  1:605600/1‑90 (MQ=255)                                                                      cTGCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAAcc    >  1:323617/1‑90 (MQ=255)                                                                        gCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCAt  >  1:659405/1‑90 (MQ=255)                                                                        gCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCAt  >  1:110235/1‑90 (MQ=255)                                                                        gCGGTTTTAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGACATTTTTTTATTTCTAAACCAt  >  1:480093/1‑90 (MQ=255)                                                                               |                                                                                   CTTACCGTTATCTGAACTTCAACCAGCTTTCTCAGTACACCGAGAAAGCCGATGGGGTGATTTTCCAGACTGCGGTTTAAAAAGTCAGCGCACGCGCTGCGCATAAACGACACAATGCCCGGTGAATGAGATTCCCGGGCATTTTTTTATTTCTAAACCAT  >  NZ_CP009273/130620‑130780

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 25 ≤ ATCG/ATCG < 32 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑