| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 832,048 | 0 | T | G | 58.3% | 20.4 / 29.1 | 24 | V81G (GTC→GGC) | hcxB | hydroxycarboxylate dehydrogenase HcXB |
| Reads supporting (aligned to +/- strand): ref base T (0/10); new base G (14/0); total (14/10) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 5.10e-07 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.57e-06 | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCG‑CATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACG > NZ_CP009273/831962‑832131 | gATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGGCAc > 2:381415/1‑90 (MQ=255)gATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGGCAc > 2:256845/1‑90 (MQ=255)gATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCAc < 2:353742/90‑1 (MQ=255) aGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGAt < 1:256845/90‑1 (MQ=255) aTGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGGGACCCTCGATGGCg > 1:301125/1‑90 (MQ=255) aTGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGGCACGCCCCATGGCg > 2:17822/1‑90 (MQ=255) aTGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGGCACCCTCGATgggg > 1:236046/1‑88 (MQ=255) aTGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGGCACCCCCGATGGCg > 2:26231/1‑90 (MQ=255) aTGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGGCACGCCCGATGGCg > 1:53224/1‑90 (MQ=255) gCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGGCCCGCCCCATGGGGATcgcg > 2:23492/1‑90 (MQ=255) cTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGGGGGCACGCTCGATGGGGGTCGCG‑CCtt > 1:475637/1‑90 (MQ=255) cAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGGGGCGGGTACGCTCCTTGGGGAGCGCG‑CAATTGGTCAg > 2:841/1‑90 (MQ=255) aCCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGGCACCCCCGATGGCGATCGCG‑CCTTTTGGGAGGTGGCgg > 2:130200/1‑90 (MQ=255) ccTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCG‑CATTTGGTCAGGTCGCGGc < 1:381415/90‑1 (MQ=255) tcatGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGGCACGCCCCATGGCGAACGCGCCATTTGG‑CAGGGCGGGGGACATGAAGCGATggg > 2:267959/1‑89 (MQ=255) ccAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCG‑CATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTgg < 1:300314/90‑1 (MQ=255) aaaaCCGTTAAAGAGGCGGGGGGGGGGGGCCCCCCCCCTGGCGGTCGCGCCATTTGG‑CAGGGCGCGGCACATGAAGCGAAGGGGCCgggg > 2:305268/1‑90 (MQ=255) gTTAAAGAGGCGGGGGCGGCGGGCACGCTCGATGGCGATCGCG‑CATTTGGTCAGGGCGCGGGACATGAAGCGATGGCGCTGGGGGTTgag > 1:73559/1‑90 (MQ=255) tAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCG‑CATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGaa < 1:17822/90‑1 (MQ=255) agagGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCG‑CATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAgc < 2:412894/90‑1 (MQ=255) cGGGGGCGGCGGTCACGCTCGATGGCGATCGCG‑CATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATc < 2:367353/90‑1 (MQ=255) gcggcgGTCACGCTCGATGGCGATCGCG‑CATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCAc < 2:73559/90‑1 (MQ=255) cggcggTCACGCTCGATGGCGATCGCG‑CATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACg < 2:299285/90‑1 (MQ=255) cggcggTCACGCTCGATGGCGATCGCG‑CATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCCGGGGATTGAGAAAGCGCATCAGCACg < 1:267959/90‑1 (MQ=255) | GATCCCAAGCTATGTACGCTCCTGGAGTCAGGGGCACCTGCAAATTAACCATCATGCCAAAACCGTTAAAGAGGCGGGGGCGGCGGTCACGCTCGATGGCGATCGCG‑CATTTGGTCAGGTCGCGGCACATGAAGCGATGGCGCTGGGGATTGAGAAAGCGCATCAGCACG > NZ_CP009273/831962‑832131 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 35 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |