| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 832,212 | 0 | T | G | 62.5% | 7.9 / 11.9 | 17 | F136V (TTT→GTT) | hcxB | hydroxycarboxylate dehydrogenase HcXB |
| Reads supporting (aligned to +/- strand): ref base T (2/4); new base G (10/0); total (13/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 8.24e-03 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.30e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
AACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCC > NZ_CP009273/832155‑832297 | aaCTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGt < 1:130200/90‑1 (MQ=255) aCTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGGTTTTCTCTATCCCCTTTTTTTGCGGGGGCggga > 1:375565/1‑88 (MQ=255) catcatATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGtt < 1:222360/53‑1 (MQ=255) catcatATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGtt > 2:222360/1‑53 (MQ=255) catcatATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGGTTTTTTCTATTCCCTTTGTTTGCGGGGGCGGGATTTCa > 2:100959/1‑90 (MQ=255) catcatATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGGTTGTCTCTATCCACTTTGTTAGCGTGGTCGGGATTCCa > 1:111472/1‑90 (MQ=255) catcatATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGGTTGTCTCTATCCACTTTGTTAGCGGGGGCGGGATTCCa > 1:161353/1‑90 (MQ=255) gCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCgcgc < 2:475637/90‑1 (MQ=255) ggTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGGTTGTCTCTATCCACTTTTTTAGCGGGGGCGGGATTCCAATGGTCGCGCCGTTcccc > 2:192070/1‑88 (MQ=255) gggCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTcgcg > 1:14220/1‑90 (MQ=255) gtgCAGCGGCGGGGGTTGTTTCTATCCACTTTTTTTGGGTGGGCGGGATTCCAATGGTCGCGCCGTTCCCCGGTCGCGGCAGCCGCTTTg > 2:434326/1‑90 (MQ=255) gtgCAGCGGCGGGGGTTGTCTCTATCCACTTTTTTTGCGTGGGCGGGATTCCAATGGGCGCGCCGTTCCCCGGGCGCGGCAGCCGCtttt > 2:155171/1‑89 (MQ=255) cAGCGGCGGGGGTTTTCTCTATCCACTTTGTTTGCGTGGGCGGGATTCCAATGGGCGCGCCGTTCCACGGGCGCGACAGCCGCTTTGGCa > 2:147221/1‑90 (MQ=255) gcggGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGGGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAAt > 1:50364/1‑90 (MQ=255) gcggGGGTTGTCTCTATCCCCTTTGTTTGCGTGGGCGGGATTCCAATGGGCGCGCCCTTCCCCGGTCGCGACAGCCGCTTTTGCACCAAt > 2:250309/1‑90 (MQ=255) gcggGGGTTGTCTCTATCCACTTTTTTAGCGTGGGCGGGATTCCAATGGGCGCGCCGTTCCACGGGCGCGACCGCCGCGTTGGCACCAAt > 2:307649/1‑90 (MQ=255) cggGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATc < 2:162274/90‑1 (MQ=255) cggGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATc < 1:434326/90‑1 (MQ=255) ggggATTGTCTCTATCCACTTTGGTAGCGTGGGCGGGATTCCAAAGGTCGCGCCGTTCCCCGGGCGCGGCAGCCGCCTTGGGACCAATcc > 2:300377/1‑90 (MQ=255) | AACTCGCATCATATCGGCCGTATCGGTTACTGGGCGGAGCAGTGTGCAGCGGCGGGGTTTGTCTCTATCCACTTTGTTAGCGTGGTCGGTATTCCAATGGTCGCGCCGTTCCACGGTCGCGACAGCCGCTTTGGCACCAATCC > NZ_CP009273/832155‑832297 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 20 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |