Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092731,536,3760AC58.3% 7.4 / 10.8 12F158V (TTT→GTT) narZnitrate reductase Z subunit alpha
Reads supporting (aligned to +/- strand):  ref base A (4/1);  new base C (0/7);  total (4/8)
Fisher's exact test for biased strand distribution p-value = 1.01e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 2.93e-03
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTTCCTGCCAGTTGGAGCGGATAAACCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCC  >  NZ_CP009273/1536303‑1536446
                                                                         |                                                                      
ccGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTTCCTGCCAGTTGGAGCGGATAAACCCGCCGCGCCCACGc                                                        <  1:8322/90‑1 (MQ=255)
                   aGACGTTAGGGGCGGCAATCAGCTGTTTTAGTTCCTGCCAGTTGGAGCGGATAACCCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGc                                     <  1:270482/90‑1 (MQ=255)
                         tAGCGGCGGCAATCAGCTGGTTTAGTTCCTGCCAGTTGGAGCGGATAAACCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACtttt                               >  1:3110/1‑90 (MQ=255)
                               cggcAATCGGCTGTTTTAGTTCCTGCCGGTTGGAGCGGATAACCCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGAt                         <  2:344949/90‑1 (MQ=255)
                               cggcAATCAGCTGGTTTAGTTCCTGCCAGTTGGAGCGGATAACCCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGAt                         <  1:341162/90‑1 (MQ=255)
                                                 tttCCTGCCAGTGGGAGCGGATAACCCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGccc       <  1:500902/89‑1 (MQ=255)
                                                 ttcccTCCCAGTGGGAGCGAATAACCCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGccc       <  2:89277/87‑1 (MQ=255)
                                                     cTGCCAGTTGGAGCGGATAAACCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGc   >  1:238879/1‑90 (MQ=255)
                                                     cTGCCAGTTGGAGCGGATAAACCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGc   >  1:362888/1‑90 (MQ=255)
                                                      tGCCAGTTGGAGCGGATAACCCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGcc  <  1:38731/90‑1 (MQ=255)
                                                      tGCCAGTTGGAGCGGATAAACCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGcc  >  1:12596/1‑90 (MQ=255)
                                                      tGCCAGTGGGAGCGGAAAACCCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGcc  <  2:12596/90‑1 (MQ=255)
                                                                         |                                                                      
CCGTAGGTTTTGATGGTCCAGACGTTAGCGGCGGCAATCAGCTGGTTTAGTTCCTGCCAGTTGGAGCGGATAAACCCGCCGCGCCCACGCACTTGTTTGTAGCTCAGGCACTTTTGCGGATCGTTCATAATCGATGCCCACGCC  >  NZ_CP009273/1536303‑1536446

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 15 ≤ ATCG/ATCG < 33 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑