Read alignment evidence...
  seq id position ref new freq score (cons/poly) reads annotation genes product
*NZ_CP0092732,254,4800AC53.8% 4.1 / 10.2 13V137G (GTG→GGG) fruAPTS fructose transporter subunit IIBC
Reads supporting (aligned to +/- strand):  ref base A (3/3);  new base C (0/7);  total (3/10)
Fisher's exact test for biased strand distribution p-value = 6.99e-02
Kolmogorov-Smirnov test that lower quality scores support variant p-value = 1.70e-01
Rejected as consensus: E-value score below prediction cutoff.
Rejected as consensus: Frequency below/above cutoff threshold.

CGACTTCTTCGGGAGTGATTGCATTACCCGCGCCAACAGAACCACGGGTTTCAACTTTCACCCACCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAGCCGCCATAAAGGTGTGTGCTACGCCAGTCGGGC  >  NZ_CP009273/2254421‑2254555
                                                           |                                                                           
cGACTTCTTCGGGAGTGATTGCATTCCCCGCGCCAACAGAACCACGGGTTTCAACTTTCACCCACCAGCCACGTTTTTTCGCTTCGGttt                                               <  2:272620/90‑1 (MQ=255)
              gTGATTGCATTCCCCGCGCCAACAGAACCACGGTTTTCAACTTTCCCCCACCAGCCACGTTTTTTCCCTTCGGTTTCAATGGCTTCAgcc                                 <  1:245543/90‑1 (MQ=255)
              gTGATTGCATTCCCCGCGCCAACAGAACCACGGGTTTCAACTTTCCCCCCCCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAgcc                                 <  1:359647/90‑1 (MQ=255)
              gTGATTGCATTCCCCGCGCCAACAGAACCACGGGTTTCAACTTTCACCCCCCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAgcc                                 <  1:355939/90‑1 (MQ=255)
              gTGATTGCATTACCCGCGCCAACAGAACCACGGGTTTCAACTTTCACCCACCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAgcc                                 >  1:201376/1‑90 (MQ=255)
              gTGATCGCATTCCCCGCGCCAACAGACCCACGGTTTTCAACTTCCCCCCACCAGCCCCGTTTTTTCGCTTCGGTTTCAATGGCTTCAgcc                                 <  2:424243/90‑1 (MQ=255)
                gATTGCATTCCCCGCCCCAACAGAACCACGGGTTTCAACTTTCCCCCACCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAgccgc                               <  1:313584/90‑1 (MQ=255)
                gATTGCATTACCCGCGCCAACAGAACCACGGGTTTCAACTTTCACCCACCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAgccgc                               >  2:69592/1‑90 (MQ=255)
                gATTGCATCACCCGCGCCACCAGAACCCCGGGTTTCAACTTTCACCCACCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAgccgc                               <  1:201777/90‑1 (MQ=255)
                           ccGCGCAAACAAACCCACGGTTTTCAACTTCCCCCCCCCACCCCCGTTTTTTCGCTTCGGTTTCAATGGCTTCAGCCGCCATAAAGgtgt                    <  2:5620/90‑1 (MQ=255)
                              cgcCCACAGAACCACGGGTTTCAACTTTCACCCACCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAGCCGCCATAAAGGtgtgtg                 >  2:163316/1‑90 (MQ=255)
                                  aaCAGAACCCCGGGTTTCAACTTTCCCCCCCCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAGCCGCCATAAAGGTGTGTGCTAc             <  1:304695/90‑1 (MQ=255)
                                             gggTTTCAACTTTCCCCCACCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAGCCGCCATAAAGGTGTGTGCTACGCCAGTCGGGc  <  1:195711/90‑1 (MQ=255)
                                                           |                                                                           
CGACTTCTTCGGGAGTGATTGCATTACCCGCGCCAACAGAACCACGGGTTTCAACTTTCACCCACCAGCCACGTTTTTTCGCTTCGGTTTCAATGGCTTCAGCCGCCATAAAGGTGTGTGCTACGCCAGTCGGGC  >  NZ_CP009273/2254421‑2254555

Alignment Legend
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 8 ≤ ATCG/ATCG < 23 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG
Unaligned base: atcg    Masked matching base: atcg    Alignment gap: ‑    Deleted base: ‑