| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,769,918 | 0 | T | G | 57.1% | 9.6 / 12.6 | 14 | R58R (CGT→CGG) | yfjY | RadC family protein |
| Reads supporting (aligned to +/- strand): ref base T (0/6); new base G (8/0); total (8/6) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 3.33e-04 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 8.55e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
GCGCGAAACAGGCGTGGCATTCACCTCCACTCAGGCTGCTCGTGACTGGCTGAAGCTGAAAATGGCGGGGCTGGAGCGTGAAGAATTTATGATGCTGTATCTGAACCAGCAGAACCAGTTGATTGCCCACGAAACCCTGTTTGCCGGTTCTATTAGCAGTACCGA > NZ_CP009273/2769840‑2770004 | gcgcGAAACAGGCGTGGCATTCACCTCCACTCAGGCTGCTCGTGACTGGCTGAAGCTGAAAATGGCGGGGCTGGAGCGTGAAGAATTTat < 1:176055/90‑1 (MQ=255) aaaCAGGCGTGGCATTCACCTCCACTCAGGCTGCTCGTGACTGGCTGAAGCTGAAAATGGCGGGGCTGGAGCGTGAAGAATTTATGATGc < 1:140203/90‑1 (MQ=255) ggCATTCACCTCCACTCAGGCTGCTCGTGACTGGCTGAAGCTGAAAATGGCGGGGCTGGGGGGGGGAGAAATTTTTATGGTGTATCTGaa > 2:200412/1‑90 (MQ=255) ccACTCAGGCTGCTCGTGACTGGCTGAAGCTGAAAATGGCGGGGCTGGGGCGGGAAGAATTTATTATGCTGTATTTGAACCAGCAGaaac > 1:444211/1‑88 (MQ=255) ggCTGCTCGTGACTGGCTGAAGCTGAAAATGGCGGGGCTGGAGCGGGAAGAATTTATGATGGTGTATTTGAACCAGCAGAACCAGTTggt > 1:450872/1‑88 (MQ=255) gctCGTGACTGGCTGAAGCTGAAAATGGCGGGGCTGGGGCGGGAAGAATTTTTGATGCTGTATCTGAACCAGCAGAACCAGTTGATTTcc > 1:486558/1‑90 (MQ=255) tGACTGGCTGAAGCTGAAAATGGCGCGGCTGGCGTCTGAAGATTCTCTGATGCTGTATCTGAACCAGCAGAACTCGTTCATTGCCCACgc < 2:333718/90‑2 (MQ=255) ggCTGAAGCTGAAAATGGCGGGGGTGGGGGGGGAAAAAATTTTGATGCTGTGTCTTAAACAGCAGAAACAGGTTATTGTCCACGAAAacc > 2:425480/1‑90 (MQ=255) gctgaagctgaaAATGGCGGGGGTGGGGGGGGGAGAAATTTTGATGGTGTATCTGAAACAGCAGAAACAGTTGATTGGCCACGAAAaccc > 2:474828/1‑89 (MQ=255) gctgaagctgaaAATGGCGGGGCTGGGGGGGGAAGAATTTTTGATGCTGTTTCTGAACCAGCAGAACCAGTTGATTGCCCACGAAAACCt > 2:45135/1‑90 (MQ=255) gaaAATGGCGGGGCTGGAGCGTGAAGAATTTATGATGCTGTATCTGAACCAGCAGAACCAGTTGATTGCCCACGAAACCCTGTTTGCCgg < 1:480468/90‑1 (MQ=255) gggCTGGAGCGTGAAGAATTTATGATGCTGTATCTGAACCAGCAGAACCAGTTGATTGCCCACGAAACCCTGTTTGCCGGTTCTATTAGc < 2:483150/90‑1 (MQ=255) ggggCGGGAAGAATTTTTGATGCTGTATCTGAACCAGCAGAACCAGTTGGTTGCCCCCGAAAACCCGTTTGCCGGGTTTTTTTACAGTAc > 2:199389/4‑90 (MQ=255) gCGTGAAGAATTTATGATGCTGTATCTGAACCAGCAGAACCAGTTGATTGCCCACGAAACCCTGTTTGCCGGTTCTATTAGCAGTACCGa < 1:425480/90‑1 (MQ=255) | GCGCGAAACAGGCGTGGCATTCACCTCCACTCAGGCTGCTCGTGACTGGCTGAAGCTGAAAATGGCGGGGCTGGAGCGTGAAGAATTTATGATGCTGTATCTGAACCAGCAGAACCAGTTGATTGCCCACGAAACCCTGTTTGCCGGTTCTATTAGCAGTACCGA > NZ_CP009273/2769840‑2770004 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 7 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |