| Read alignment evidence... | |||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| seq id | position | ref | new | freq | score (cons/poly) | reads | annotation | genes | product | ||
| * | NZ_CP009273 | 2,794,496 | 0 | T | G | 54.5% | 9.3 / 10.6 | 12 | V58G (GTG→GGG) | nrdI | class Ib ribonucleoside‑diphosphate reductase assembly flavoprotein NrdI |
| Reads supporting (aligned to +/- strand): ref base T (1/4); new base G (6/0); total (8/4) | |||||||||||
| Fisher's exact test for biased strand distribution p-value = 1.52e-02 | |||||||||||
| Kolmogorov-Smirnov test that lower quality scores support variant p-value = 4.28e-03 | |||||||||||
| Rejected as consensus: E-value score below prediction cutoff. | |||||||||||
| Rejected as consensus: Frequency below/above cutoff threshold. | |||||||||||
CGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCGCGGCGTTATTGCTTCTGGTAATCGCAACTTTGGTG > NZ_CP009273/2794409‑2794585 | cGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTggggcggggc > 2:186312/1‑90 (MQ=255) gCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGcc < 1:389756/90‑1 (MQ=255) aaTGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGAGGCGGGACGGCTGGCGCCGCGCCACgc > 1:340829/1‑89 (MQ=255) gATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGGGCCCCGACAGGGAATTCGCTTTTTAAACGACGAGCACAACCGGGCGtt > 2:85898/1‑90 (MQ=255) gTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGGGGCCCGACAGGGAATTTGCCTTTTTAACCACGAGCACAACCGGGCGGTGCtt > 2:491148/1‑90 (MQ=255) gCCCTCTTACGGCGGCGGCGGTACGGCTGGGGCGGGGCCCCGGCAGGGAAATCGCTTTTTTAACGGCGGGCACAACCGGGCGGTTCTTcg > 2:389557/1‑90 (MQ=255) gCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGGGCCACGACAGGGAATTCGCTTTTTAAACGACGAGCACAACCGGGCGGTGCTTcg > 2:130850/1‑90 (MQ=255) cccTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTcgc < 1:274934/90‑1 (MQ=255) ccTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGCAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTcgcg < 1:337453/90‑1 (MQ=255) tctTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGGTGCTTCgcggc > 1:489533/1‑90 (MQ=255) ggcggcggGACGGCTGGGGCGGGGCCACGACAGGGAATTCGCTTTTTTAACGACGAGCACAACCGGGGGGTGCTTCGCGGGGGTATTGCt > 2:204998/1‑90 (MQ=255) cggcggTACGGCTGGGGCGGGGGCACGACAGGGAATTCGCTTTTTAAAAGACGGGCACAACCGGGGGTTTCTTCGCGGCGGTATttcttc > 2:84175/1‑90 (MQ=255) cgcgGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCGCGGCGTTATTGCTTCTGGTAATCGCAACtt < 2:417915/90‑1 (MQ=255) tGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCGCGGCGTTATTGCTTCTGGTAATCGCAACTTTGGTg < 1:385540/90‑1 (MQ=255) | CGCTCAATGAGCGGGAACGGATTCAGGTAGACGAGCCTTACATCCTGATCGTGCCCTCTTACGGCGGCGGCGGTACGGCTGGCGCGGTGCCACGACAGGTAATTCGCTTTTTAAACGACGAGCACAACCGGGCGTTGCTTCGCGGCGTTATTGCTTCTGGTAATCGCAACTTTGGTG > NZ_CP009273/2794409‑2794585 |
| Alignment Legend |
|---|
Aligned base mismatch/match (shaded by quality score): ATCG/ATCG < 3 ≤ ATCG/ATCG < 5 ≤ ATCG/ATCG < 6 ≤ ATCG/ATCG < 17 ≤ ATCG/ATCG < 41 ≤ ATCG/ATCG |
Unaligned base: atcg Masked matching base: atcg Alignment gap: ‑ Deleted base: ‑ |